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Children's Research Institute CRISPR Cas9 Injection Request Form Name/Lab: Date: APN: Lab Location: Mouse Room: Gene (Exon): PI Approval: Subledger/IDR number: What is the concentration of sRNA that
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Open the cri-cas9-mouse-injection-formdocx file.
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Start with filling in the necessary details such as the date, your name, and contact information.
03
Provide information about the mouse strain and genotype as required.
04
Specify the details of the CRISPR/Cas9 system being used for injection, including the target gene and guide RNA sequence.
05
Fill out the injection parameters such as the injection volume and concentration of CRISPR/Cas9 components.
06
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Researchers or scientists working on CRISPR/Cas9-mediated gene editing in mice.
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Animal facility managers or personnel responsible for coordinating mouse injection experiments.
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Laboratory technicians or assistants involved in carrying out mouse injections using the CRISPR/Cas9 system.
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The cri-cas9-mouse-injection-formdocx is a document used for the reporting and approval of CRISPR-Cas9 mouse injection experiments, detailing the experimental procedures and methodologies.
Researchers and laboratories conducting CRISPR-Cas9 mouse injection experiments are required to file the cri-cas9-mouse-injection-formdocx.
To fill out the cri-cas9-mouse-injection-formdocx, researchers must provide detailed information regarding the experiment, including the purpose, methods, and any relevant animal welfare considerations.
The purpose of the cri-cas9-mouse-injection-formdocx is to ensure proper documentation and compliance with ethical standards for experiments involving genetic modifications in mice using CRISPR technology.
The form must report information such as the objective of the experiment, methods to be used, mouse strain details, potential risks, and the measures taken for animal welfare.
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cas9 crispr mrna sgrna childrens plasmid transfection t7e1 dna 1ug nuclease amplicons idr apn subledger exon mediated mammalian assay alleles surveyor sequencing submitting restriction ug hr mutated validation mutations cloned transient cleavage construct

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