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This form is used for the submission of samples for analysis at the Mass Spectrometry Facility. It includes sections for customer identification, sample identification, analysis instructions, and

How to fill out protein peptide mass spectrometry

How to fill out PROTEIN & PEPTIDE MASS SPECTROMETRY SAMPLE SUBMISSION FORM

1. Obtain the PROTEIN & PEPTIDE MASS SPECTROMETRY SAMPLE SUBMISSION FORM from the appropriate laboratory website or contact.
2. Fill in your contact information including name, email, and phone number in the designated sections.
3. Specify the type of protein or peptide being submitted, including any relevant details like concentration and volume.
4. Indicate the desired analysis type, whether it’s protein identification, quantification, or characterization.
5. List any specific conditions or requirements for the sample, such as temperature or storage recommendations.
6. Provide any relevant background information about the samples, including source and any prior treatments.
7. Review all filled information for accuracy and completeness.
8. Sign and date the form, if required, before submission.

Who needs PROTEIN & PEPTIDE MASS SPECTROMETRY SAMPLE SUBMISSION FORM?

1. Researchers working with protein or peptide samples who require mass spectrometry analysis.
2. Laboratories that perform protein and peptide analysis to aid in research and development.
3. Pharmaceutical companies developing new drugs and need to analyze protein interactions.
4. Academic institutions conducting studies in biochemistry or related fields.

People Also Ask about

What is mass spectrometry for protein and peptide analysis?

In protein mass spectrometry, the masses of different proteins and peptides are determined by measuring the m/z (mass-to-charge) ratio of their gas-phase ions. Mass spectrometers first convert protein molecules into gas-phase ions using an ion source. Next, a mass analyzer separates ionized analytes based on m/z ratio.

What is the protein concentration required for mass spec?

As most MS techniques, native MS is quite sensitive and usually requires protein concentration in the range of 1-25 mM. Final volumes of the sample solution can be as low as 15 ml, but usually have to be larger prior to buffer exchange that is required for MS analysis.

How much protein needed for mass spectrometry?

For a label-free quantitative proteomics experiment typically 20 µg of total protein per sample are required. This amount corresponds to approximately 300,000 cells. For a phosphoproteomic analysis about 500 µg total protein per sample are needed.

How do you prepare a mass spectrometry sample?

Sample Preparation Protocol for Open Access MS Dissolve the sample in any organic solvent (e.g., DCM, CHCl3, EtOAc, MeCN, MeOH) or H2O to a concentration of 1mg/mL. Take 100uL of this solution and dilute it with 1mL of either methanol, acetonitrile or water (or any combination of these solvents).

How much protein do you need for NMR?

Typically 300 ul (with specialized NMR tubes) to 600 ul of sample is needed. Protein concentrations for well-behaved systems should be above 150 uM for structural studies, but lower concentrations may be used for other non-structural studies.

How much protein do you need for mass spectrometry?

For a label-free quantitative proteomics experiment typically 20 µg of total protein per sample are required. This amount corresponds to approximately 300,000 cells. For a phosphoproteomic analysis about 500 µg total protein per sample are needed.

How much sample is needed for mass spec?

The amount of sample required depends on the mass spectrometry service to be performed. In general, 1 mL of a 10 ppm sample or 1 mg in solid form is enough for all applications.

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What is PROTEIN & PEPTIDE MASS SPECTROMETRY SAMPLE SUBMISSION FORM?

The PROTEIN & PEPTIDE MASS SPECTROMETRY SAMPLE SUBMISSION FORM is a document used to provide essential information about samples submitted for mass spectrometry analysis of proteins and peptides.

Who is required to file PROTEIN & PEPTIDE MASS SPECTROMETRY SAMPLE SUBMISSION FORM?

Researchers, laboratories, and institutions that are submitting samples for protein and peptide mass spectrometry analysis are required to file this form.

How to fill out PROTEIN & PEPTIDE MASS SPECTROMETRY SAMPLE SUBMISSION FORM?

To fill out the form, provide accurate details about the sample identity, description, concentration, and any special handling instructions, ensuring all sections are completed as per guidelines.

What is the purpose of PROTEIN & PEPTIDE MASS SPECTROMETRY SAMPLE SUBMISSION FORM?

The purpose of the form is to standardize the submission process, ensuring that all necessary information is communicated to facilitate accurate and efficient analysis of the samples.

What information must be reported on PROTEIN & PEPTIDE MASS SPECTROMETRY SAMPLE SUBMISSION FORM?

The form must report information such as sample names, concentrations, sample types, submitted quantities, and any relevant experimental conditions or requirements.