Get the free Reverse transcriptase PCR - Fisher Scientific

Get Form

Show details

REVERSE TRANSCRIPTASE PCR* BACKWARDS TO THE FUTURE Got RNA? Need a copy of its parent? Reverse transcription allows just that! Combine this process with PCR, and you've got reverse transcriptase PCR

We are not affiliated with any brand or entity on this form

Get, Create, Make and Sign reverse transcriptase pcr

Edit your reverse transcriptase pcr form online

Type text, complete fillable fields, insert images, highlight or blackout data for discretion, add comments, and more.

Add your legally-binding signature

Draw or type your signature, upload a signature image, or capture it with your digital camera.

Share your form instantly

Email, fax, or share your reverse transcriptase pcr form via URL. You can also download, print, or export forms to your preferred cloud storage service.

How to edit reverse transcriptase pcr online

Ease of Setup

pdfFiller User Ratings on G2

Ease of Use

pdfFiller User Ratings on G2

Follow the steps below to benefit from a competent PDF editor:

1

Create an account. Begin by choosing Start Free Trial and, if you are a new user, establish a profile.

2

Upload a file. Select Add New on your Dashboard and upload a file from your device or import it from the cloud, online, or internal mail. Then click Edit.

3

Edit reverse transcriptase pcr. Rearrange and rotate pages, add and edit text, and use additional tools. To save changes and return to your Dashboard, click Done. The Documents tab allows you to merge, divide, lock, or unlock files.

4

Get your file. When you find your file in the docs list, click on its name and choose how you want to save it. To get the PDF, you can save it, send an email with it, or move it to the cloud.

pdfFiller makes dealing with documents a breeze. Create an account to find out!

Uncompromising security for your PDF editing and eSignature needs

Your private information is safe with pdfFiller. We employ end-to-end encryption, secure cloud storage, and advanced access control to protect your documents and maintain regulatory compliance.

Learn more about security & compliance

How to fill out reverse transcriptase pcr

How to fill out reverse transcriptase PCR:

01

Start by setting up a clean and sterile workspace. Ensure that all materials, reagents, and equipment are readily available.

02

Prepare the reaction mix by combining the following components in a sterile microcentrifuge tube:

2.1

RNA template: The sample you wish to analyze. It can be RNA isolated from cells, tissues, or any other source.

2.2

Reverse transcriptase enzyme: This enzyme converts RNA into complementary DNA (cDNA). Add an appropriate amount of reverse transcriptase enzyme to the reaction mix based on manufacturer's instructions.

2.3

Nucleotides: These are the building blocks of DNA. Add a mix of deoxynucleotide triphosphates (dNTPs) to the reaction mix.

2.4

Buffer: Reverse transcriptase PCR requires a specific buffer that provides optimal conditions for the reaction. Add the buffer to the reaction mix.

2.5

Primers: Add gene-specific primers to the reaction mix. These primers bind to the RNA template and allow the reverse transcriptase enzyme to start the cDNA synthesis.

2.6

RNase inhibitor (optional): If not using purified RNA, add an RNase inhibitor to protect RNA from degradation during the reaction.

03

Mix the components gently by pipetting up and down, making sure all reagents are thoroughly mixed. Be careful not to introduce any air bubbles.

04

Place the reaction mix in a thermal cycler or PCR machine, which will provide the necessary temperature changes for the reaction to occur.

05

Set the thermal cycling parameters optimized for reverse transcriptase PCR. Typically, this involves an initial denaturation step at a high temperature (e.g., 95°C) to denature RNA and activate the reverse transcriptase enzyme. This is followed by a series of temperature cycles, including annealing and extension steps that allow for cDNA synthesis and amplification.

06

Run the reverse transcriptase PCR reaction according to the specified thermal cycling parameters. Monitor the progress using appropriate detection methods such as real-time PCR or gel electrophoresis.

07

Once the reaction is complete, analyze the resulting cDNA by the desired method, such as gene expression analysis, cloning, or sequencing.

Who needs reverse transcriptase PCR:

01

Researchers studying gene expression: Reverse transcriptase PCR is a valuable tool for studying gene expression patterns. It allows the conversion of RNA into cDNA, which can be amplified and analyzed to examine which genes are active in a particular tissue or under specific experimental conditions.

02

Diagnostic laboratories: Reverse transcriptase PCR plays a crucial role in diagnosing certain infectious diseases, especially those caused by RNA viruses. By converting viral RNA into cDNA, specific sequences can be amplified and detected, aiding in the diagnosis and monitoring of viral infections.

03

Pharmaceutical companies: Reverse transcriptase PCR is used during the drug development process to investigate the effects of potential compounds on gene expression. It helps determine the impact of drugs on specific target genes, enabling researchers to evaluate their effectiveness and potential side effects.

In conclusion, reverse transcriptase PCR is a versatile technique used in various scientific fields to convert RNA into complementary DNA and study gene expression. Its applications range from basic research to clinical diagnostics and drug development.

Fill form : Try Risk Free

Rate the form

4.0

Satisfied

59 Votes

For pdfFiller’s FAQs

Below is a list of the most common customer questions. If you can’t find an answer to your question, please don’t hesitate to reach out to us.

How do I execute reverse transcriptase pcr online?

pdfFiller has made it easy to fill out and sign reverse transcriptase pcr. You can use the solution to change and move PDF content, add fields that can be filled in, and sign the document electronically. Start a free trial of pdfFiller, the best tool for editing and filling in documents.

How do I edit reverse transcriptase pcr on an iOS device?

Use the pdfFiller app for iOS to make, edit, and share reverse transcriptase pcr from your phone. Apple's store will have it up and running in no time. It's possible to get a free trial and choose a subscription plan that fits your needs.

How do I complete reverse transcriptase pcr on an iOS device?

pdfFiller has an iOS app that lets you fill out documents on your phone. A subscription to the service means you can make an account or log in to one you already have. As soon as the registration process is done, upload your reverse transcriptase pcr. You can now use pdfFiller's more advanced features, like adding fillable fields and eSigning documents, as well as accessing them from any device, no matter where you are in the world.

What is reverse transcriptase pcr?

Reverse Transcriptase PCR (RT-PCR) is a type of PCR technique used to amplify and detect RNA rather than DNA.

Who is required to file reverse transcriptase pcr?

Researchers and scientists working with RNA samples are typically required to perform reverse transcriptase PCR.

How to fill out reverse transcriptase pcr?

To fill out a reverse transcriptase PCR, one must first convert RNA to complementary DNA using reverse transcriptase enzyme before proceeding with the PCR amplification.

What is the purpose of reverse transcriptase pcr?

The purpose of reverse transcriptase PCR is to amplify and detect RNA molecules, allowing for the study of gene expression and RNA viruses.

What information must be reported on reverse transcriptase pcr?

The results of the RT-PCR amplification, including the presence or absence of the target RNA sequence, must be reported.

Fill out your reverse transcriptase pcr online with pdfFiller!

pdfFiller is an end-to-end solution for managing, creating, and editing documents and forms in the cloud. Save time and hassle by preparing your tax forms online.

Get started now