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Http://2018.BTT.abstract SUBMISSION FORM available in PDF from: http://2018.ebtt.org/abstract (For the use by the Scientific Committee only!) Paper No. Date receivedDecision by the Scientific Committee:

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1. Prepare the electroporation buffer by mixing the appropriate components according to the protocol.
2. Choose the appropriate electroporation cuvette or plate and ensure it is clean and dry.
3. Thaw the cells to be transfected and wash them with the electroporation buffer.
4. Resuspend the cells in the electroporation buffer at the desired concentration.
5. Add the DNA or RNA to be transfected into the cell suspension and mix gently.
6. Transfer the cell mixture into the electroporation cuvette or plate and make sure there are no air bubbles.
7. Place the electroporation cuvette or plate into the electroporation device and set the desired parameters (voltage, pulse width, etc.).
8. Apply the electroporation pulse by activating the device.
9. Immediately transfer the electroporated cells into a pre-warmed culture medium.
10. Incubate the cells at the appropriate temperature and time for recovery and expression of the transfected genes.
11. Analyze the transfected cells for successful gene delivery and expression.

Who needs electroporation based technologies and?

1. Researchers working in molecular biology or genetic engineering who need to introduce foreign genetic material into cells for various applications.
2. Scientists studying gene function and regulation who require a method to manipulate gene expression and analyze the consequences.
3. Pharmaceutical companies developing gene therapies or vaccines that rely on efficient delivery of genetic material into target cells.
4. Biotechnology companies involved in the production of recombinant proteins or other biotechnological products that require genetically modified cell lines.
5. Agricultural researchers and breeders seeking to improve crops or livestock through genetic engineering techniques.
6. Medical professionals exploring electroporation-based medical treatments, such as cancer therapy or tissue regeneration.
7. Forensic scientists utilizing electroporation for DNA profiling and analysis in criminal investigations.

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What is electroporation based technologies and?

Electroporation based technologies are techniques that utilize electric fields to increase the permeability of cell membranes, allowing for the introduction of various substances such as drugs, DNA, or other molecules into the cells.

Who is required to file electroporation based technologies and?

Researchers, companies, or organizations that develop or utilize electroporation technologies for therapeutic, diagnostic, or research purposes are generally required to file relevant documentation.

How to fill out electroporation based technologies and?

To fill out electroporation based technologies documentation, one needs to provide detailed information about the technology, its applications, safety data, and any relevant research findings.

What is the purpose of electroporation based technologies and?

The purpose of electroporation based technologies is to enhance the delivery of therapeutic agents into cells, thereby improving efficacy in treatments, especially in fields like gene therapy and cancer treatment.

What information must be reported on electroporation based technologies and?

Information that must be reported includes the technology description, intended use, efficacy data, safety assessment, and compliance with regulatory standards.