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BMC Microbiology Research article Boomed Central Open Access Sublingual bacterial colonization profiles correlate with gingival tissue gene expression Panos N Papapanou×1, Jan H Behle1, Moritz Kebschull1,Romania

How to fill out subgingival bacterial colonization profiles

How to fill out subgingival bacterial colonization profiles:

1. Obtain a sterile periodontal probe and gently insert it into the subgingival pockets to collect samples of the bacterial biofilm.
2. Transfer the collected biofilm samples into an appropriate transport medium or solution for preservation.
3. Label each sample with a unique identifier, ensuring accurate tracking and analysis.
4. Prepare the collected samples for microbiological analysis by employing techniques such as polymerase chain reaction (PCR), DNA sequencing, or culturing.
5. Analyze the microbiological data obtained, identifying and quantifying the different bacterial species present in the subgingival biofilm.
6. Record the results in a standardized format or database, including the bacterial species identified and their relative abundances.
7. Interpret the findings and assess the overall subgingival bacterial colonization profile to determine its clinical significance.
8. Share the results with the relevant healthcare professionals involved in the patients' dental care, such as periodontists or general dentists.

Who needs subgingival bacterial colonization profiles:

1. Periodontists: Subgingival bacterial colonization profiles provide valuable insights into the specific bacterial species responsible for periodontal disease. This information helps periodontists tailor treatment plans and monitor the success of interventions.
2. General dentists: Understanding the subgingival bacterial colonization profiles aids general dentists in identifying patients at a higher risk of developing periodontal disease or those who require additional preventive measures.
3. Researchers: Subgingival bacterial colonization profiles contribute to scientific knowledge about the interactions between oral microbiota and various dental and systemic diseases. Researchers use this information to study disease mechanisms, develop new interventions, and evaluate the effectiveness of different therapies.

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What is subgingival bacterial colonization profiles?

Subgingival bacterial colonization profiles refer to the composition and distribution of bacteria found in the subgingival (below the gum line) region of the oral cavity. These profiles provide insights into the types and quantities of bacteria present in the periodontal pockets, which can help in diagnosing and treating periodontal diseases.

Who is required to file subgingival bacterial colonization profiles?

Dentists, periodontists, or oral healthcare professionals are typically responsible for obtaining subgingival bacterial colonization profiles from patients. These profiles are not specifically filed, but rather used for diagnostic and treatment purposes.

How to fill out subgingival bacterial colonization profiles?

Subgingival bacterial colonization profiles are not filled out like traditional forms. Instead, they are obtained through the collection of subgingival plaque samples during periodontal examinations. These samples are then analyzed using molecular techniques or culture-based methods to identify and quantify the bacteria present.

What is the purpose of subgingival bacterial colonization profiles?

The purpose of subgingival bacterial colonization profiles is to assess the microbial composition and abundance in the periodontal pockets of patients. This information helps in diagnosing and managing periodontal diseases, as different bacteria can be associated with varying levels of disease progression. It also aids in the selection of appropriate antibiotic treatments and targeted therapeutic interventions.

What information must be reported on subgingival bacterial colonization profiles?

Subgingival bacterial colonization profiles typically report the types of bacterial species or groups present in the subgingival plaque samples, along with their relative abundances or quantities. Some profiles may also provide additional information, such as antibiotic resistance patterns.