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Antibody Human Cytokine Array 2 Quantitative measurement of 20 Human cytokines Patent Pending Technology User Manual (Version Oct 07) Cat # QAHCYT2 Biotech, Inc. We Provide You With Excellent Protein

How to fill out quantibody human cytokine array

How to Fill Out Quantibody Human Cytokine Array:

1. Ensure that you have all the necessary materials and reagents required for the assay, including the Quantibody Human Cytokine Array Kit, sample diluent, and the array slide.
2. Start by preparing your samples. This may involve collecting blood or other biological fluids and processing them according to the kit instructions. Dilute the samples if necessary using the provided sample diluent.
3. Thaw and equilibrate the array slide to room temperature for at least 15 minutes.
4. Carefully remove the array slide from the packaging, avoiding touching the printed side.
5. Add the diluted samples or controls to the sample wells on the array slide. Ensure that you apply the appropriate volume to each well, as stated in the kit instructions.
6. Incubate the array slide at the recommended temperature and duration as instructed in the kit manual. This step allows for the binding of cytokines present in the samples to the array slide.
7. After the incubation, wash the array slide gently with the wash buffer provided. Follow the protocol closely to ensure proper removal of unbound substances.
8. Apply the detection antibody mix to the array slide, making sure to cover each well adequately.
9. Incubate the array slide again as directed in the kit instructions, allowing the detection antibodies to bind to the cytokines captured on the array.
10. Perform a second wash step to remove unbound detection antibodies.
11. Apply the HRP-streptavidin working solution provided in the kit to the array slide, allowing it to bind to the detection antibodies.
12. After the final incubation, wash the array slide one last time to remove any excess streptavidin.
13. Apply the substrate solution to the array slide and incubate for the recommended time until spots begin to appear.
14. Stop the reaction by rinsing the array slide with distilled water and carefully blotting it dry.
15. Finally, scan the array slide using an appropriate microarray scanner, following the recommended settings provided by the manufacturer. Capture clear and high-resolution images of the array spots.
16. Analyze the obtained data using the software or analysis tools recommended by the kit manufacturer. Quantify the cytokine levels based on the spot intensity and compare them across samples.

Who Needs Quantibody Human Cytokine Array?

1. Researchers in the field of immunology or molecular biology who aim to study cytokine expression profiles in human samples can benefit from using the Quantibody Human Cytokine Array. It provides a comprehensive and efficient approach for simultaneous detection and quantification of multiple cytokines in a single sample.
2. Pharmaceutical companies and drug developers can utilize this array to evaluate the effect of potential drug candidates on cytokine expression levels. This information can help in understanding drug mechanisms and assessing their potential therapeutic applications.
3. Healthcare professionals and clinicians may find the Quantibody Human Cytokine Array useful for monitoring cytokine profiles in patients with inflammatory or immune-related diseases. By measuring cytokine levels, it could aid in disease diagnosis, prognosis, and treatment response assessment.

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What is quantibody human cytokine array?

Quantibody Human Cytokine Array is a high-throughput multiplex ELISA based assay for simultaneous quantitative measurement of multiple cytokines in a single sample.

Who is required to file quantibody human cytokine array?

Researchers, scientists, or labs conducting studies or experiments involving cytokine analysis may be required to file Quantibody Human Cytokine Array.

How to fill out quantibody human cytokine array?

Quantibody Human Cytokine Array is typically filled out by adding the sample to the designated wells on the array chip, incubating, and then following the provided protocol for detection and analysis.

What is the purpose of quantibody human cytokine array?

The purpose of Quantibody Human Cytokine Array is to provide a comprehensive analysis of multiple cytokines in a single sample, allowing for the study of immune response, inflammation, and various diseases.

What information must be reported on quantibody human cytokine array?

The information reported on Quantibody Human Cytokine Array includes the levels of different cytokines present in the sample being analyzed.

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