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Lab 8. Extracting DNA from Your Cells Name Lab Section Team No. Date Prelab Assignment 1. Before coming to lab, read all parts of this lab handout. 2. Answer the Prelab Questions 1 8 on pages 1 2

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Gather necessary equipment:

1. Test tubes
2. Pipettes
3. DNA extraction buffer
4. Enzymes (such as proteinase K)
5. Ethanol
6. Centrifuge

Start by obtaining the sample:

1. Choose the source of DNA (e.g., blood, cheek cells, plant material).
2. Use sterile swabs or needles to collect the sample and place it in a clean test tube.

Add DNA extraction buffer:

1. Carefully add the DNA extraction buffer to the test tube containing the sample.
2. Ensure that the buffer covers the sample fully.

Incubate the sample:

1. Place the test tube in a water bath or a heat block at the recommended temperature.
2. Incubate for a specified duration to allow the buffer to break down the cells and release the DNA.

Enzymatic digestion:

1. Add the appropriate enzymes (e.g., proteinase K) to the test tube.
2. These enzymes help break down proteins and contaminants present in the sample.

Mix and centrifuge:

1. Gently mix the contents of the test tube to ensure proper enzyme distribution.
2. Centrifuge the mixture to separate solid debris from the liquid DNA-containing supernatant.

Precipitate the DNA:

1. Transfer the supernatant to a new clean test tube.
2. Slowly add cold ethanol to the supernatant while gently mixing.

Collect the DNA:

1. Use a pipette to carefully collect the visible threads of DNA that start to form.
2. Place the collected DNA in a separate clean test tube.

Wash and store the DNA:

1. Rinse the collected DNA with cold ethanol to remove impurities.
2. Allow the DNA to air dry or use a vacuum concentrator.
3. Finally, store the extracted DNA in a freezer or refrigerator until further use.

Who needs extracting DNA from your?

1. Researchers and scientists working in fields such as genetics, genomics, and molecular biology.
2. Forensic experts trying to identify individuals based on DNA samples.
3. Medical professionals conducting genetic testing for purposes like diagnosing diseases or determining paternity.
4. Agriculture specialists involved in crop improvement and DNA fingerprinting.
5. Wildlife conservationists studying animal populations and biodiversity through DNA analysis.
6. Genealogists and individuals interested in tracing their ancestry using DNA testing.
7. Biotechnology companies developing new drug therapies or genetically modified organisms.
8. Environmental scientists studying microbial communities or assessing pollution levels.
9. Archaeologists examining ancient DNA to understand human history and migration patterns.
10. Veterinary professionals analyzing DNA to diagnose genetic disorders in animals.

Note: The steps provided in the answer are a general guideline and may vary depending on the specific DNA extraction protocol or kit being used. It is important to follow the instructions provided by the manufacturer or consult a laboratory professional for accurate and reliable results.

For pdfFiller’s FAQs

What is extracting dna from your?

Extracting DNA from your is the process of isolating DNA molecules from a sample, such as blood or tissue, for further analysis or testing.

Who is required to file extracting dna from your?

Individuals or researchers who are conducting DNA extractions are required to file extracting dna from your.

How to fill out extracting dna from your?

To fill out extracting dna from your, provide accurate information about the DNA extraction process, including the sample source and extraction method.

What is the purpose of extracting dna from your?

The purpose of extracting dna from your is to obtain genetic material for various scientific, medical, or forensic purposes.

What information must be reported on extracting dna from your?

Information such as the sample source, extraction method, and any relevant findings or results must be reported on extracting dna from your.

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