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1NEXT GENERATION SEQUENCING REQUISITION http://www.upmcgenomecenter.com Genome Center UPMC.edu p : 412 623 7155 5560 Center Ave Pittsburgh PA 15232 Oath L Tatum, PhD HELD/CC(ABB) DirectorORDERING
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01
Prepare the DNA sample by isolating it from the desired source material.
02
Quantify the DNA concentration using a fluorometer or spectrophotometer.
03
Dilute the DNA sample to the desired concentration.
04
Prepare the DNA library by fragmenting the DNA, adding adapters, and amplifying it.
05
Quantify the DNA library using a fluorometer or qPCR.
06
Cluster the DNA library onto a flow cell and perform sequencing using a next generation sequencing platform.
07
Analyze the sequencing data to obtain the desired information.

Who needs 1 next generation sequencing?

01
Scientists and researchers who are studying genetic variations and mutations.
02
Biologists and geneticists who are interested in understanding the genomic composition of organisms.
03
Medical professionals who are diagnosing genetic disorders and diseases.
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Pharmaceutical companies who are developing personalized medicine based on genetic profiles.
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Forensic investigators who are analyzing DNA evidence.
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Agricultural researchers who are improving crop yield and breeding programs through genetic analysis.
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Next generation sequencing (NGS) is a method used for sequencing DNA or RNA that allows for the rapid and efficient analysis of genetic material.
Researchers, scientists, and laboratories conducting genetic sequencing experiments may be required to file NGS data as part of their research or regulatory requirements.
NGS data can be generated using specialized equipment and software, and the results can be analyzed using bioinformatics tools to interpret the genetic information.
The purpose of NGS is to identify and analyze genetic variations, mutations, and gene expression profiles in various biological samples for research, diagnostic, or clinical purposes.
NGS data typically includes information on the genetic sequence of the DNA or RNA sample, quality control metrics, and any identified genetic variants or mutations.
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