Get the free Targeted knock-in mice expressing the oxidase-fixed form ... - Nature

ARTICLE https://doi.org/10.1038/s4146701912565zOPENTargeted knocking mice expressing the oxidase form of Antoine oxidoreductase favor tumor growth 1234567890():, ;Term Susan 1,6, Dress Ehirchiou2,3,6,

How to fill out targeted knock-in mice expressing

How to fill out targeted knock-in mice expressing

1. Obtain the targeting vector for the gene of interest that will be knocked-in.
2. Design the primers for PCR amplification of the homology arms of the targeting vector.
3. Isolate mouse embryonic stem (ES) cells that will be used for gene targeting.
4. Electroporate the ES cells with the targeting vector and select for positive clones using antibiotic resistance.
5. Perform PCR or Southern blot analysis to confirm successful integration of the targeting vector.
6. Inject the targeted ES cells into blastocysts to generate chimeric mice.
7. Mate chimeric mice with wild-type mice to obtain offspring with the targeted knock-in allele.
8. Perform genotyping to identify mice with the desired knock-in allele.

Who needs targeted knock-in mice expressing?

1. Researchers studying the function of a specific gene in mice.
2. Scientists investigating disease mechanisms or therapeutic targets in mice.
3. Pharmaceutical companies developing mouse models for drug discovery and testing.
4. Biotechnology companies developing genetically modified mice for research purposes.

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What is targeted knock-in mice expressing?

Targeted knock-in mice are expressing a specific gene that has been inserted into a specific location in the genome.

Who is required to file targeted knock-in mice expressing?

Researchers or institutions conducting experiments involving targeted knock-in mice are required to file the necessary paperwork.

How to fill out targeted knock-in mice expressing?

To fill out targeted knock-in mice expressing, researchers must provide detailed information about the gene being inserted, the method used for insertion, and the expected outcomes of the experiment.

What is the purpose of targeted knock-in mice expressing?

The purpose of targeted knock-in mice expressing is to study the function of specific genes and their role in disease development.

What information must be reported on targeted knock-in mice expressing?

Researchers must report details about the gene being inserted, the targeted location in the genome, the methods used for insertion, and the expected outcomes.