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Name: Chemistry 118 Laboratory University of Massachusetts Boston Beers Law LEARNING GOALS: 1. Become familiar with the concept of concentration and molarity. 2. Become familiar with making dilutions
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How to fill out lab 4 beers law

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To fill out lab 4 for Beer's Law, follow these steps:

01
Start by gathering the necessary materials and equipment required for the experiment. This may include a spectrophotometer, cuvettes, different concentrations of a solution, and a light source.
02
Prepare the solutions with varying concentrations that will be used in the experiment. Make sure to label each solution accordingly to avoid confusion.
03
Set up the spectrophotometer according to the manufacturer's instructions. This usually involves calibrating the instrument and adjusting the wavelength to the desired range.
04
Take a blank solution (a solution without the analyte) and place it in a cuvette. Insert the cuvette into the spectrophotometer and set the absorbance to zero. This step is crucial to properly account for background absorbance.
05
Take the first prepared solution and measure its absorbance. Place the cuvette containing the solution into the spectrophotometer and record the absorbance reading. Repeat this step for each of the prepared solutions.
06
Plot a graph with absorbance (Y-axis) against concentration (X-axis) for the different solutions. This graph is known as a calibration curve and will help determine the relationship between absorbance and concentration.
07
Analyze the graph to determine the equation of the line that best represents the relationship between absorbance and concentration. This equation is typically in the form of y = mx + b, where y is absorbance, x is concentration, m is the slope, and b is the y-intercept.
08
Use the equation obtained from the calibration curve to calculate the concentration of an unknown solution. Measure the absorbance of the unknown solution using the spectrophotometer and plug this value into the equation.
09
Calculate and record the concentration of the unknown solution based on the equation. This step allows you to quantitatively determine the concentration of the analyte in the unknown solution.

Who needs lab 4 Beer's Law?

Lab 4 for Beer's Law is typically completed by students studying chemistry or related disciplines. It is a fundamental laboratory experiment that introduces the concept of Beer's Law, which relates the concentration of a solution to its absorbance. Understanding the principles behind Beer's Law is important for individuals working in fields such as analytical chemistry, pharmaceuticals, environmental sciences, and biochemistry, as it can be used to determine the concentration of unknown solutions and assess the intensity of a substance's color or absorption properties. This lab is commonly performed in educational settings to reinforce theoretical knowledge and develop practical laboratory skills.
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Lab 4 Beers Law is a scientific experiment that demonstrates the relationship between the concentration of a solution and the amount of light it absorbs.
Students in a chemistry laboratory class are typically required to perform and file lab 4 Beers Law.
To fill out lab 4 Beers Law, students need to measure the absorbance of different solutions at various concentrations and plot a graph to determine the concentration of an unknown solution.
The purpose of lab 4 Beers Law is to understand the relationship between the concentration of a solution and its absorbance of light, which is important in fields like chemistry and biochemistry.
On lab 4 Beers Law, students must report the concentrations of the solutions, the absorbance readings, and the calculated concentration of the unknown solution.
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