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HEALTH SERVICESIQCL A B O(EML4ALK) ORATORIES LUNG ALK IOC REQUEST Format ADVANCED DIAGNOSTIC CS UCL Advanced DiagnosticsLabelledAddress: HSL AD, First Floor, 60 Whitfield Street, London, W1T 4EU 1st
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How to fill out lung alk eml4-alk ihc
How to fill out lung alk eml4-alk ihc
01
To fill out lung alk eml4-alk ihc, follow these steps:
02
Prepare the necessary materials such as a tissue sample from the lung, a microscope slide, and the reagents for the immunohistochemistry (IHC) staining.
03
Fix the tissue sample in a formalin solution to preserve its structure.
04
Embed the fixed tissue in paraffin wax to facilitate sectioning.
05
Cut thin sections of the tissue on a microtome.
06
Mount the sections onto a microscope slide.
07
Perform antigen retrieval by heating the sections in a retrieval buffer.
08
Apply the primary antibody specific to ALK (anaplastic lymphoma kinase) and EML4-ALK fusion protein to the sections.
09
Incubate the slides to allow the antibody to bind to the target protein.
10
Wash the slides to remove any unbound antibodies.
11
Apply a secondary antibody labeled with a detection system, such as a chromogen or a fluorophore, to visualize the primary antibody's binding.
12
Counterstain the sections with a contrasting dye to enhance the visualization of the stained cells.
13
Mount a coverslip onto the slides using a mounting medium.
14
Observe the stained sections under a microscope and analyze the presence of ALK and EML4-ALK fusion protein.
15
Document the results and interpret them accordingly.
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Note: It is essential to follow standard laboratory protocols and guidelines for safety and accuracy during the entire process.
Who needs lung alk eml4-alk ihc?
01
Lung ALK EML4-ALK IHC is typically performed for individuals who have been diagnosed with lung cancer and are suspected of having the ALK (anaplastic lymphoma kinase) gene rearrangement. This genetic rearrangement, commonly known as EML4-ALK fusion, is found in a subset of non-small cell lung cancer (NSCLC) cases. Conducting the IHC test helps in identifying patients who may benefit from ALK-targeted therapies, as these drugs specifically target the abnormal ALK protein and inhibit its activity. Therefore, individuals with lung cancer who are being considered for targeted therapy or whose treatment plans may involve ALK inhibitors may require lung ALK EML4-ALK IHC for diagnostic and treatment decision-making purposes.
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What is lung alk eml4-alk ihc?
Lung alk eml4-alk ihc is a test used to detect the EML4-ALK gene rearrangement in lung cancer patients.
Who is required to file lung alk eml4-alk ihc?
Medical professionals and laboratories performing the test are required to file lung alk eml4-alk ihc.
How to fill out lung alk eml4-alk ihc?
The test results and patient information must be accurately filled out on the required form provided by the testing facility.
What is the purpose of lung alk eml4-alk ihc?
The purpose of lung alk eml4-alk ihc is to guide treatment decisions for lung cancer patients based on their genetic profile.
What information must be reported on lung alk eml4-alk ihc?
The test results, patient demographics, and any other relevant information related to the EML4-ALK gene rearrangement must be reported.
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