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HEALTH SERVICESIQCL A B O(EML4ALK) ORATORIES LUNG ALK IOC REQUEST Format ADVANCED DIAGNOSTIC CS UCL Advanced DiagnosticsLabelledAddress: HSL AD, First Floor, 60 Whitfield Street, London, W1T 4EU 1st

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How to fill out lung alk eml4-alk ihc

01

Step 1: Start by preparing the lung tissue samples for IHC staining.

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Step 2: Fix the samples in formalin and embed them in paraffin blocks.

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Step 3: Cut thin sections of the tissue samples using a microtome.

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Step 4: Mount the tissue sections on glass slides and dry them overnight.

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Step 5: Dewax the sections by immersing them in xylene and rehydrate them using a series of graded alcohols.

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Step 6: Retrieve the antigen by boiling the sections in a citrate buffer solution.

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Step 7: Block the non-specific binding sites on the tissue sections using a blocking buffer.

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Step 8: Incubate the sections with an EML4-ALK primary antibody.

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Step 9: Wash the sections to remove any unbound primary antibody.

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Step 10: Apply a secondary antibody conjugated with an enzyme or fluorochrome.

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Step 11: Visualize the antibody-antigen complex using the appropriate detection system.

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Step 12: Counterstain the tissue sections with a suitable counterstain.

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Step 13: Mount coverslips on the slides using a mounting medium.

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Step 14: Observe and analyze the stained lung tissue sections under a microscope.

Who needs lung alk eml4-alk ihc?

01

Lung ALK (EML4-ALK) IHC is needed for the diagnosis and management of patients with non-small cell lung cancer (NSCLC).

02

It helps in identifying patients with ALK gene rearrangements, which can be targeted with specific ALK inhibitors for personalized treatment.

03

This test is typically recommended for patients with NSCLC who may benefit from targeted therapy.

04

It assists in selecting the appropriate treatment strategy and monitoring the response to ALK inhibitor therapy.

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What is lung alk eml4-alk ihc?

lung alk eml4-alk ihc is a test used to detect the fusion of the ALK gene with the EML4 gene in lung cancer cells.

Who is required to file lung alk eml4-alk ihc?

Pathologists and oncologists are typically required to perform and report lung alk eml4-alk ihc.

How to fill out lung alk eml4-alk ihc?

To fill out lung alk eml4-alk ihc, a pathologist or oncologist must analyze the lung cancer tissue sample for ALK gene rearrangement.

What is the purpose of lung alk eml4-alk ihc?

The purpose of lung alk eml4-alk ihc is to identify patients with non-small cell lung cancer who may benefit from targeted therapy with ALK inhibitors.

What information must be reported on lung alk eml4-alk ihc?

The report for lung alk eml4-alk ihc must include the results of the ALK gene rearrangement analysis in lung cancer tissue.

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