Get the free Tangential flow affinity ultrafiltration - European Patent Office - EP 0229696 A1. B...

Europaisches Patent amt European Patent Office GO Publication number:PATENT Date of filing: 09.01.87Priority: 10.01.86 GB 8600582Date of publication of application: 22.07.87 Bulletin 87/30Designated

How to fill out tangential flow affinity ultrafiltration

How to fill out tangential flow affinity ultrafiltration

1. Set up the tangential flow system by connecting the feed reservoir to the ultrafiltration unit.
2. Prepare the affinity ligand by immobilizing it onto the membrane surface or using pre-coated membranes.
3. Adjust the pressure and flow rate settings according to the specifications of the ultrafiltration unit.
4. Prime the system with a buffer solution to remove any air bubbles and ensure proper flow.
5. Load the sample containing the target molecule into the feed reservoir.
6. Start the tangential flow affinity ultrafiltration process and monitor the performance of the system.
7. Collect the filtrate, which contains the unbound molecules, in a separate collection vessel.
8. Once the desired separation is achieved, stop the process and clean the system thoroughly for reuse.

Who needs tangential flow affinity ultrafiltration?

1. Tangential flow affinity ultrafiltration is commonly used in biological and pharmaceutical research and manufacturing processes.
2. Researchers and scientists involved in protein purification, antibody screening, drug discovery, and biomolecule characterization often require this technique.
3. It is also used in the biotechnology industry for the separation and purification of therapeutic proteins, enzymes, and other biomolecules.
4. The technique is beneficial for individuals and organizations seeking to isolate specific molecules from complex mixtures and achieve high-purity samples.

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What is tangential flow affinity ultrafiltration?

Tangential flow affinity ultrafiltration is a technique used for separating and purifying biomolecules based on their affinity for a specific ligand immobilized on a membrane surface.

Who is required to file tangential flow affinity ultrafiltration?

Researchers and laboratories using tangential flow affinity ultrafiltration for purification of biomolecules are required to document and report their processes.

How to fill out tangential flow affinity ultrafiltration?

To fill out tangential flow affinity ultrafiltration, one must provide detailed information about the process parameters, materials used, experimental conditions, and outcomes of the purification process.

What is the purpose of tangential flow affinity ultrafiltration?

The purpose of tangential flow affinity ultrafiltration is to isolate and purify biomolecules based on their specific interactions with a ligand, allowing for high purity and yield.

What information must be reported on tangential flow affinity ultrafiltration?

The information to be reported on tangential flow affinity ultrafiltration includes details of the biomolecules, ligands used, purification methods, equipment, and results obtained during the process.