Get the free Dulbecco''s Modified Eagle Medium (D-MEM) (1X), liquid (high glucose)

SAFETY DATA SHEET 1. Identification of the substance/mixture and of the company/undertaking Identification of the substance/preparation Product code Product name 12430120 Dulbecco is Modified Eagle

How to fill out dulbeccos modified eagle medium

How to fill out Dulbecco's modified eagle medium:

1. Ensure that you are working in a sterile environment, preferably inside a laminar flow hood.
2. Gather all the necessary equipment and reagents required for preparing the medium. This may include Dulbecco's modified eagle medium powder, sterile distilled water, antibiotics, and pH adjusters.
3. Calculate the amount of medium you need based on the number of plates or flasks you will be using. Follow the manufacturer's instructions for the correct dilution ratio.
4. Weigh the appropriate amount of Dulbecco's modified eagle medium powder and transfer it to a clean and sterile container.
5. Gradually add the sterile distilled water to the powder while stirring gently to dissolve it completely. Make sure there are no clumps or undissolved particles.
6. Check the pH of the medium using a pH meter or indicator strips. Adjust the pH to the desired range, usually around 7.4, by adding small amounts of pH adjusters such as sodium bicarbonate or hydrochloric acid. Stir gently after each addition and recheck the pH until it reaches the desired value.
7. If using antibiotics, prepare a separate stock solution following the manufacturer's instructions. Add the appropriate volume of the antibiotic stock solution to the medium and mix well.
8. Sterilize the medium by using an autoclave or a sterile filtration system. Autoclave at 121°C for 15 minutes or filter through a 0.22 μm filter to remove any potential contaminants.
9. Allow the medium to cool down to room temperature before use. This may take several hours if using an autoclave.
10. Once cooled, the Dulbecco's modified eagle medium is ready to be used for cell culture experiments.

Who needs Dulbecco's modified eagle medium?

1. Researchers and scientists involved in cell culture experiments often require Dulbecco's modified eagle medium. It is a widely used basal medium for the growth and maintenance of many types of mammalian cells in vitro.
2. Cell culture facilities in academic institutions, pharmaceutical companies, and biotechnology laboratories extensively use this medium to support the proliferation and survival of various cell lines.
3. Dulbecco's modified eagle medium is particularly useful for culturing adherent cells, suspension cells, and primary cells. It provides the necessary nutrients, vitamins, amino acids, and growth factors essential for cellular growth and proliferation.
4. Additionally, this medium is commonly used in a variety of applications such as virus production, gene expression studies, cytotoxicity assays, and drug screening.
5. Researchers studying cell biology, immunology, cancer research, and regenerative medicine often rely on Dulbecco's modified eagle medium as their choice of culture medium.

For pdfFiller’s FAQs

What is dulbeccos modified eagle medium?

Dulbecco's Modified Eagle Medium (DMEM) is a commonly used cell culture medium for the cultivation of a wide variety of mammalian cells.

Who is required to file dulbeccos modified eagle medium?

Researchers and scientists working with mammalian cell cultures are required to use and file DMEM for their experiments.

How to fill out dulbeccos modified eagle medium?

DMEM is typically filled out by adding the necessary supplements and adjusting the pH to the desired levels before use in cell culture experiments.

What is the purpose of dulbeccos modified eagle medium?

The purpose of DMEM is to provide essential nutrients and a suitable environment for the growth and survival of mammalian cells in laboratory settings.

What information must be reported on dulbeccos modified eagle medium?

Information such as the composition of the medium, any additional supplements added, pH levels, and batch number may need to be reported on DMEM.

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