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SAFETY DATA SHEET 1. Identification of the substance/mixture and of the company/undertaking Identification of the substance/preparation Product code Product name 4425101 2X Ligation Buffer Company/Undertaking
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How to fill out 2x ligation buffer

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How to fill out 2x ligation buffer:

01
Start by preparing all the necessary materials and reagents, including the 2x ligation buffer, DNA fragments to be ligated, and ligase enzyme.
02
Thaw the 2x ligation buffer if it is stored at a low temperature. Gently mix the buffer to ensure it is well-mixed before use.
03
Calculate the volume of 2x ligation buffer needed based on the amount of DNA fragments and the recommended ratio provided in the protocol. Ensure that the final concentration of the ligation buffer is at a suitable level for the ligation reaction.
04
Add the calculated volume of 2x ligation buffer to a clean, sterile microcentrifuge tube.
05
Add the DNA fragments to be ligated to the same microcentrifuge tube containing the ligation buffer. Mix gently by pipetting up and down or by lightly tapping the tube.
06
If using a ligase enzyme, add the appropriate amount according to the manufacturer's instructions to the same tube. Mix gently again.
07
Incubate the ligation reaction mix at the recommended temperature and time specified in the protocol. This allows the ligase enzyme to catalyze the ligation reaction between the DNA fragments.
08
After the incubation period, the 2x ligation buffer and ligated DNA fragments can be used for downstream applications, such as transformation or further experimentation.

Who needs 2x ligation buffer:

01
Researchers and scientists who are working in molecular biology or genetics laboratories often require 2x ligation buffer for various molecular cloning experiments.
02
2x ligation buffer is used in DNA ligation reactions, which join together DNA fragments, such as vector and insert DNA, to create recombinant DNA molecules.
03
It is especially useful in molecular cloning techniques, such as plasmid construction, gene cloning, or creating recombinant DNA libraries.
04
Laboratories working on genetic engineering, gene expression analysis, or genetic modification frequently rely on 2x ligation buffer for their experiments.
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2x ligation buffer is a solution used in molecular biology to promote the ligation of DNA fragments.
Researchers and scientists working in the field of molecular biology are required to prepare and use 2x ligation buffer.
To prepare 2x ligation buffer, you need to mix specific components according to the protocol provided.
The purpose of 2x ligation buffer is to optimize the DNA ligation reaction between DNA fragments.
The components and concentrations of the reagents used in the 2x ligation buffer must be reported.
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