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LTIMSDS1 MATERIAL SAFETY DATA SHEET Page 1 of 8 Revised 1/02/03 E. COLI STRAIN BL21AI IN CCMB80 BUFFER Replaces (None) NITROGEN CORPORATION Printed 1/02/03 MSDS ID: 500315 1. PRODUCT AND COMPANY INFORMATION
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How to fill out coli strain bl21ai in
To fill out coli strain bl21ai, you will need the following materials:
01
A culture of the bl21ai strain of E. coli
02
Appropriate growth medium
03
Sterile culture tubes or plates
04
Sterile pipettes
05
Incubator set to the appropriate temperature
06
Sterilization equipment
6.1
Start by preparing the growth medium according to the manufacturer's instructions. This may involve dissolving a powder in water or sterilizing a liquid medium. Ensure that the medium is at the correct temperature before proceeding.
6.2
Sterilize all equipment by autoclaving or using appropriate sterilization methods.
6.3
Transfer a small amount of the bl21ai culture to a sterile culture tube or plate, using a sterile pipette. Be sure to use aseptic techniques to avoid contamination.
6.4
Add the prepared growth medium to the culture tube or plate, using a sterile pipette. The volume of medium added should depend on the specific requirements of your experiment or protocol.
6.5
Mix the culture and medium gently to ensure even distribution and contact between the bacteria and the growth medium.
6.6
Seal the culture tube or plate to maintain sterility and prevent contamination. This may involve using a cap, parafilm, or another suitable method.
6.7
Place the culture tube or plate in the incubator set to the appropriate temperature for the bl21ai strain. The recommended temperature is typically around 37 degrees Celsius for E. coli.
6.8
Allow the bacteria to grow and replicate in the incubator for the specified amount of time. This may vary depending on the desired cell density for your experiment or protocol.
6.9
Once the incubation period is complete, your bl21ai strain culture will be ready for use in your specific application, whether it be protein expression, gene cloning, or other genetic manipulation techniques.
Who needs coli strain bl21ai in?
01
Researchers in the field of molecular biology often need the bl21ai strain of E. coli for various applications. This strain is widely used for protein expression, as it carries specific genetic modifications that enhance protein production.
02
Scientists who work with recombinant DNA technologies may require the bl21ai strain for cloning or expressing specific genes of interest.
03
Biotechnology companies and pharmaceutical companies may utilize the bl21ai strain for large-scale production of recombinant proteins.
In conclusion, understanding how to properly fill out coli strain bl21ai and knowing who needs it is crucial for researchers in the fields of molecular biology, genetic engineering, and protein expression. Following the outlined steps and utilizing the bl21ai strain can greatly contribute to successful experiments and advancements in various biological applications.
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What is coli strain bl21ai in?
Coli strain bl21ai is a commonly used bacterial strain in molecular biology research.
Who is required to file coli strain bl21ai in?
Researchers and scientists working with coli strain bl21ai are required to file the necessary documentation.
How to fill out coli strain bl21ai in?
The process of filling out coli strain bl21ai documentation involves providing detailed information about the strain and the purpose of its usage.
What is the purpose of coli strain bl21ai in?
Coli strain bl21ai is often used for protein expression in recombinant DNA technology.
What information must be reported on coli strain bl21ai in?
Information such as the origin of the strain, genetic modifications, experimental protocols, and safety precautions must be reported.
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