Get the free Quant-iT PicoGreen dsDNA Reagent and Kits - Thermo Fisher Scientific

Final Biotech Telephone: 800.558.4511 Fax:414.357.4518 www.dynalbiotech.com Gel Documentation Form B17 SSP Unitary B17 SSP 003 Kit Name: Lot#: Batch#: Exp. Date: Tested by: Institution: Name: Sample

How to fill out quant-it picogreen dsdna reagent

How to fill out quant-it picogreen dsdna reagent:

Prepare the reagent:

1. Thaw the quant-it picogreen dsdna reagent at room temperature.
2. Gently mix the reagent by inverting the bottle a few times.

Prepare the DNA sample:

1. Dilute the DNA sample to a suitable concentration for analysis. This usually involves adding a certain volume of the DNA sample to a known volume of a suitable buffer.
2. Mix the diluted DNA sample thoroughly by gentle pipetting or vortexing.

Prepare the working solution:

1. In a separate tube, prepare the working solution by adding the appropriate volume of the quant-it picogreen dsdna reagent.
2. Calculate the amount of reagent based on the number of samples being analyzed and any additional controls required.

Add the working solution to the DNA sample:

1. Add the prepared working solution to the diluted DNA sample.
2. Mix thoroughly by pipetting or vortexing to ensure proper mixing of the reagent and DNA.

Incubate and measure the fluorescence:

1. Incubate the sample and working solution mixture at room temperature in the dark for a specific duration, as recommended by the quant-it picogreen dsdna reagent protocol.
2. After the incubation period, measure the fluorescence using a fluorometer or a plate reader that is compatible with the quant-it picogreen dsdna reagent.

Who needs quant-it picogreen dsdna reagent?

1. Researchers in molecular biology: The quant-it picogreen dsdna reagent is commonly used by researchers working in molecular biology to accurately quantify small amounts of dsDNA. It is a sensitive and reliable method for DNA quantification, making it essential for various applications such as PCR, cloning, sequencing, and other molecular biology experiments.
2. Biotechnology and pharmaceutical companies: These industries often utilize the quant-it picogreen dsdna reagent in their research and development processes. They require precise DNA quantification for tasks like gene expression analysis, sample purity assessment, and quality control of DNA samples used in various biotechnological and pharmaceutical applications.
3. Forensic laboratories: Forensic scientists use the quant-it picogreen dsdna reagent to measure the amount of DNA obtained from crime scene evidence. DNA quantification is crucial in forensic analysis, determining the viability of DNA samples for further analysis, and ensuring accurate and reliable results in criminal investigations.

In summary, anyone working in molecular biology, biotechnology, pharmaceuticals, or forensic sciences may need the quant-it picogreen dsdna reagent to accurately measure and quantify DNA samples for their respective applications.

For pdfFiller’s FAQs

What is quant-it picogreen dsdna reagent?

Quant-iT PicoGreen dsDNA reagent is a fluorescent dye used for quantifying double-stranded DNA in molecular biology applications.

Who is required to file quant-it picogreen dsdna reagent?

Researchers and scientists conducting experiments or studies involving DNA quantification are required to use and file quant-iT PicoGreen dsDNA reagent.

How to fill out quant-it picogreen dsdna reagent?

To fill out quant-iT PicoGreen dsDNA reagent, you need to follow the protocol provided by the manufacturer, which typically involves mixing the reagent with a DNA sample and measuring the fluorescence using a compatible instrument.

What is the purpose of quant-it picogreen dsdna reagent?

The purpose of quant-iT PicoGreen dsDNA reagent is to accurately quantify the amount of double-stranded DNA present in a sample, which is essential for various molecular biology experiments and research studies.

What information must be reported on quant-it picogreen dsdna reagent?

The information reported on quant-iT PicoGreen dsDNA reagent includes the concentration of dsDNA in the sample, the volume of reagent used, any dilutions made, and the instrument settings used for fluorescence measurement.

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