Get the free Acid-PhenolChloroform, pH 4

Certificate of Analysis Kit/Product Name: Acid-Phenol:Chloroform, pH 4.5 Catalog Number: 9720, AM9720 Note: The prefix AM will be added to all existing Albion product catalog numbers, beginning October

How to fill out acid-phenolchloroform ph 4

How to fill out acid-phenolchloroform pH 4:

1. Find a suitable work area that is equipped with necessary safety measures, such as a fume hood or a well-ventilated space.
2. Wear appropriate personal protective equipment, including gloves, goggles, and a lab coat, to ensure safety during the procedure.
3. Prepare the acid-phenolchloroform pH 4 solution by combining equal volumes of phenol, chloroform, and the appropriate acid solution, typically pH 4 (such as acetic acid or formic acid).
4. Mix the components thoroughly by gentle inversion, avoiding vigorous shaking to prevent emulsification.
5. Allow the mixture to separate into distinct phases, typically an organic (bottom) phase and an aqueous (top) phase. This separation may take a few minutes.
6. Transfer the desired phase (usually the upper aqueous phase) to a new sterile tube, using a pipette or a glass Pasteur pipette to avoid contamination.
7. Repeat the mixing and separation steps, if necessary, to obtain the desired purity or quantity of the specific phase required for your experiment or application.
8. Store the obtained phase appropriately, following the recommended storage conditions and precautions mentioned on the acid-phenolchloroform pH 4 solution label or in the accompanying documentation.

Who needs acid-phenolchloroform pH 4:

1. Molecular biologists: Acid-phenolchloroform pH 4 is commonly used in molecular biology techniques, such as DNA and RNA extraction, to denature proteins, lipids, and other components while isolating nucleic acids.
2. Researchers in the field of genetics: Acid-phenolchloroform pH 4 is essential in genetic studies to purify DNA or RNA from various biological samples, enabling further analysis, such as sequencing or genotyping.
3. Scientists conducting gene expression studies: Acid-phenolchloroform pH 4 plays a vital role in isolating high-quality RNA from cells or tissues, preserving the integrity of RNA molecules for downstream applications like reverse transcription and gene expression analysis.
4. Diagnostic laboratories: Acid-phenolchloroform pH 4 is required for isolating nucleic acids from clinical specimens in diagnostics labs, aiding in the detection of infectious agents or genetic abnormalities.
5. Biotechnology companies: Acid-phenolchloroform pH 4 is utilized in the production and purification of recombinant proteins, where the separation of nucleic acids from protein samples is crucial to obtain a pure and functional product.

For pdfFiller’s FAQs

What is acid-phenolchloroform ph 4?

Acid-phenolchloroform ph 4 is a reagent commonly used in molecular biology for DNA extraction.

Who is required to file acid-phenolchloroform ph 4?

Researchers and scientists working with DNA samples are typically required to use and file acid-phenolchloroform ph 4.

How to fill out acid-phenolchloroform ph 4?

To fill out acid-phenolchloroform ph 4, follow the specific instructions provided by the manufacturer or protocol.

What is the purpose of acid-phenolchloroform ph 4?

The purpose of acid-phenolchloroform ph 4 is to aid in the extraction and purification of DNA from biological samples.

What information must be reported on acid-phenolchloroform ph 4?

Information such as the DNA sample source, extraction protocol used, and any modifications made to the standard procedure should be reported on acid-phenolchloroform ph 4.

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