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OP RPM Form 110 (Rev. 01/2005)Date Notice Posted November 19, 2015 (OP RPM use only)UNIVERSITY OF HAWAII NOTICE OF SOLE SOURCE Item(s) to be purchased:High Performance Liquid Chromatography SystemDescription
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How to fill out high performance liquid chromatography

01
Prepare the HPLC system by flushing all the necessary solvents through the system.
02
Prepare the sample by dissolving it in the appropriate solvent.
03
Inject the sample into the HPLC system using an autosampler.
04
Set the appropriate parameters such as flow rate, column temperature, and detector wavelength.
05
Start the HPLC run and monitor the chromatogram to analyze the separation of components.
06
Interpret the results based on peak shape, retention times, and peak areas.

Who needs high performance liquid chromatography?

01
Scientists and researchers in the fields of analytical chemistry, pharmaceuticals, environmental science, and biochemistry who need to separate, identify, and quantify compounds in a mixture.
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High performance liquid chromatography (HPLC) is a technique used to separate and identify components in a mixture based on their different interactions with a stationary phase and a mobile phase.
Scientists, researchers, and professionals in the fields of chemistry, biology, pharmaceuticals, and environmental science are typically required to file high performance liquid chromatography.
To fill out high performance liquid chromatography, one must prepare the sample, set up the HPLC equipment, inject the sample, run the analysis, and interpret the results.
The purpose of high performance liquid chromatography is to separate, identify, and quantify components in a mixture for analytical or preparative purposes.
Information such as the sample name, peak retention times, peak areas, and peak identities must be reported on high performance liquid chromatography.
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