Get the free ExpiFectamine 293 Transfection Kit - Thermo Fisher ScientificExpi293F Cell Transfect...
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Transit PRO Transfection Reagent for Protein
Production in Expi293F Cells
Quick Reference Protocol
Instructions for MIR 5720, 5730, 5740 and 5750SPECIFICATIONS
StorageStore Transit PRO Reagent tightly
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How to fill out expifectamine 293 transfection kit
How to fill out expifectamine 293 transfection kit
01
Thaw the Expifectamine 293 reagent at room temperature.
02
Dilute the DNA in Opti-MEM medium and mix gently.
03
Add the Expifectamine 293 reagent to the diluted DNA mixture and mix gently.
04
Incubate the transfection complex at room temperature for 15-30 minutes.
05
Add the transfection complex to the cells and incubate for desired time period.
Who needs expifectamine 293 transfection kit?
01
Researchers working with HEK 293 cells who want to efficiently transfect them with DNA.
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What is expifectamine 293 transfection kit?
The Expifectamine 293 Transfection Kit is a specialized reagent system used for efficiently introducing nucleic acids into eukaryotic cells, specifically using the Expi293 cell line for high-level protein expression.
Who is required to file expifectamine 293 transfection kit?
Researchers and laboratories engaged in cell transfection processes using the Expifectamine 293 kit are required to file necessary documentation regarding its use.
How to fill out expifectamine 293 transfection kit?
To fill out the Expifectamine 293 transfection kit, carefully follow the included protocol, which outlines the preparation of the transfection mix, cell handling, and necessary documentation for reporting experiments.
What is the purpose of expifectamine 293 transfection kit?
The purpose of the Expifectamine 293 Transfection Kit is to facilitate the transfection of DNA or RNA into cultured cells, enabling researchers to study gene expression and produce recombinant proteins.
What information must be reported on expifectamine 293 transfection kit?
Users must report the experimental conditions, including the nucleic acids used, cell line, transfection efficiency, and any relevant outcomes or findings related to their transfection experiments.
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