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TEPZZZ4ZZB_T(19)(11)EP 2 304 030 B1EUROPEAN PATENT SPECIFICATION(12)(45) Date of publication and mention(51) Int Cl.:C12N 15/11 (2006.01)of the grant of the patent: 25.11.2015 Bulletin 2015/48C12N

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How to fill out recombinant dna constructs and

01

Gather all necessary materials including plasmid DNA, desired gene(s), restriction enzymes, DNA ligase, and competent cells.

02

Digest the plasmid DNA and desired gene(s) with the appropriate restriction enzymes to generate compatible ends for ligation.

03

Purify the digested DNA fragments to remove any contaminants that could interfere with ligation.

04

Mix the digested fragments in the presence of DNA ligase to facilitate the ligation of the gene(s) into the plasmid DNA.

05

Transform the ligated DNA into competent cells using a suitable method such as heat shock or electroporation.

06

Select transformed cells using antibiotic selection or other markers to ensure successful incorporation of the gene(s) into the plasmid DNA.

07

Verify successful recombinant DNA constructs through sequencing or other molecular biology techniques.

Who needs recombinant dna constructs and?

01

Researchers in the fields of genetics, molecular biology, biotechnology, and medicine often need recombinant DNA constructs to study gene function, create genetically modified organisms, produce recombinant proteins, or develop gene therapy treatments.

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What is recombinant dna constructs and?

Recombinant DNA constructs are artificially created DNA sequences that combine genetic material from multiple sources.

Who is required to file recombinant dna constructs and?

Researchers and institutions working with recombinant DNA constructs are required to file the necessary documentation.

How to fill out recombinant dna constructs and?

Recombinant DNA constructs forms can typically be filled out online or in paper format, following specific guidelines provided by the relevant regulatory body.

What is the purpose of recombinant dna constructs and?

The purpose of recombinant DNA constructs is to create new genetic combinations for research or practical applications.

What information must be reported on recombinant dna constructs and?

Information such as the DNA sequences used, the sources of genetic material, the purpose of the construct, and safety measures must be reported.

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