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Environmental Toxicology Laboratory University of Saskatchewan Saskatoon, Saskatchewan, Canada, S7N 5B3 Effective Date 05/31/18SOP: UofSETLEDNA20 Revision: Page: 1 of 11Replaces SOP: n/environmental
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How to fill out metabarcoding of zooplankton communities

01
Collect water samples from the desired location
02
Filter the samples to concentrate the zooplankton
03
Extract DNA from the filtered samples
04
Amplify the DNA using PCR with specific primers targeting the barcode region
05
Sequence the amplified DNA using high-throughput sequencing technology
06
Analyze the sequencing data to identify and quantify the zooplankton species present

Who needs metabarcoding of zooplankton communities?

01
Researchers studying aquatic ecosystems and biodiversity
02
Environmental agencies monitoring water quality and ecosystem health
03
Conservationists assessing the impact of human activities on zooplankton populations
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Metabarcoding of zooplankton communities is a technique used to identify and quantify the different species of zooplankton present in a sample by analyzing their DNA.
Researchers, scientists, and organizations studying aquatic ecosystems may be required to file metabarcoding of zooplankton communities.
Metabarcoding of zooplankton communities is typically filled out by conducting DNA extractions, PCR amplifications, and sequencing of the DNA to identify the species present.
The purpose of metabarcoding of zooplankton communities is to understand the biodiversity and community structure of zooplankton in aquatic environments.
Information such as the species identified, their relative abundance, sampling location, and environmental factors may need to be reported on metabarcoding of zooplankton communities.
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