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CHEM 8L, UCSCBinderExperiment 3 TLC Analysis of Spinach Pigments Background Reading / Watching Morris Chapters 9 11 (filtration, extraction, and drying) Videos on spinach extraction and TLC on CHEM

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How to fill out thin layer chromatography lab

01

Prepare the developing chamber and pour the solvent mixture into the chamber.

02

Mark a baseline on the TLC plate with a pencil or marker.

03

Spot the solution to be analyzed onto the baseline using a micro capillary or pipette.

04

Place the TLC plate into the developing chamber and allow the solvent to move up the plate.

05

Remove the TLC plate from the chamber once the solvent front reaches near the top of the plate.

06

Allow the plate to dry and visualize the separated components using UV light or a developing agent.

07

Measure the Rf values of the spots to analyze the compounds present in the sample.

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What is thin layer chromatography lab?

Thin layer chromatography lab is a technique used to separate and analyze mixtures of chemicals by passing them through a thin layer of adsorbent material.

Who is required to file thin layer chromatography lab?

Scientists, researchers, and students in the field of chemistry are typically required to perform and file thin layer chromatography labs.

How to fill out thin layer chromatography lab?

To fill out a thin layer chromatography lab, one must follow specific protocols and procedures outlined in the lab manual, including preparing the sample, applying it to the plate, developing the plate, and analyzing the results.

What is the purpose of thin layer chromatography lab?

The purpose of thin layer chromatography lab is to separate and identify components of a mixture based on their different affinities for the adsorbent material.

What information must be reported on thin layer chromatography lab?

The information reported on a thin layer chromatography lab typically includes the compounds tested, their retention factor values, and any visual observations made during the analysis.

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