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Standard Operating Procedure Center for Systems Neurogenetic of Addiction (CSA) Cocaine Loco motor Sensitization Assay (Sens v1.0)STANDARD OPERATING PROCEDURE Document Number: SOPCSNABPC Sense v1.0Page
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01
Set up the locomotor activity chambers and the video tracking system.
02
Acclimate the mice to the testing room for at least 1 hour.
03
Inject the mice with a saline solution to habituate them to the injection procedure.
04
Administer the cocaine injection at the desired dose, typically done once daily for several days.
05
Place the mice in the locomotor activity chambers and record their movement over a specified period of time.
06
Analyze the data to determine the locomotor sensitization response to cocaine.

Who needs cocaine locomotor sensitization assay?

01
Researchers studying drug addiction and the effects of cocaine on behavior.
02
Pharmaceutical companies developing new medications for substance abuse.
03
Medical professionals studying the mechanisms of drug-induced sensitization.
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Cocaine locomotor sensitization assay is a preclinical animal behavior test used to study the long-lasting neuroadaptations associated with repeated exposure to cocaine.
Researchers and scientists conducting studies involving cocaine-induced locomotor sensitization are required to file the assay results.
The assay is typically filled out by recording the locomotor activity of animals after repeated cocaine exposure and calculating sensitization indices.
The purpose of the assay is to understand the behavioral changes and neural adaptations that occur in response to repeated cocaine exposure, which are indicative of addiction.
The assay report should include details of the experimental setup, drug dosages used, locomotor activity measurements, and sensitization indices.
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