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Romero et al. BMC Genomics (2018) 19:703 https://doi.org/10.1186/s1286401850822RESEARCH ARTICLEOpen AccessComparison of RNA seq and microarray platforms for splice event detection using a cross-platform
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How to fill out custom selected reference genes

01
Select a set of candidate reference genes based on literature review or previous experimental data
02
Design primers for the selected reference genes using a suitable software tool
03
Perform validation experiments to determine the stability of the reference genes under experimental conditions
04
Use the most stable reference genes for normalization of gene expression data

Who needs custom selected reference genes?

01
Researchers working on gene expression analysis
02
Scientists studying specific biological pathways or processes
03
Laboratories performing quantitative PCR experiments
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Custom selected reference genes are specific genes that are chosen by an individual or organization to serve as a baseline for gene expression analysis in a particular study or experiment.
Researchers, scientists, or individuals conducting gene expression analysis studies who choose to use custom selected reference genes are required to file this information.
Custom selected reference genes can be filled out by providing the name of the chosen genes, their specific functions, and any other relevant information about why they were selected for the study.
The purpose of custom selected reference genes is to provide a stable and consistent baseline for gene expression analysis, ensuring accurate and reliable results in the study or experiment.
The information reported on custom selected reference genes should include the names of the chosen genes, their functions, and the rationale for selecting them as reference genes for the study.
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