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ESTABLISHING A SPONGE HYBRID OMA FOR THE IN VITRO PRODUCTION OF A MARINE NATURAL PRODUCT by Cassady Dougan Thesis Submitted to the Faculty of Charles E. Schmidt College of Science In Partial Fulfillment

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How to fill out establishing a sponge hybridoma

01

Obtain a sponge tissue sample from a live sponge.

02

Isolate sponge cells from the tissue sample using enzymatic digestion.

03

Fuse the isolated sponge cells with myeloma cells to create hybridoma cells.

04

Culture the hybridoma cells in a suitable media to allow them to proliferate.

05

Screen the hybridoma cells to identify clones that produce the desired antibody.

06

Expand and maintain the selected hybridoma clone for antibody production.

Who needs establishing a sponge hybridoma?

01

Researchers and scientists studying the immune system of sponges or looking to produce specific antibodies from sponge cells.

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What is establishing a sponge hybridoma?

Establishing a sponge hybridoma involves creating immortalized cell lines from specific immune cells (B cells) that produce antibodies against a particular antigen.

Who is required to file establishing a sponge hybridoma?

Researchers and scientists working on developing antibody-producing cell lines from sponges are required to file establishing a sponge hybridoma.

How to fill out establishing a sponge hybridoma?

To fill out establishing a sponge hybridoma, researchers need to provide detailed information on the sponge species, antigen of interest, cell culture techniques, and antibody production methods.

What is the purpose of establishing a sponge hybridoma?

The purpose of establishing a sponge hybridoma is to produce a sustainable source of specific antibodies for research, diagnostics, and potential therapeutic applications.

What information must be reported on establishing a sponge hybridoma?

Information such as sponge species used, antigen specificity, cell line characteristics, antibody production protocols, and validation data must be reported on establishing a sponge hybridoma.

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