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Microbiology (1997), 143,32513262Printed in Great BritainSequencing and muteness of genes from the biosynthetic gene cluster of Saccharopolyspora Eritrea that are involved in Lmycarose and Ddesosamine
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01
Design primer sequences for the target gene to be sequenced or mutated.
02
Perform polymerase chain reaction (PCR) to amplify the DNA fragment containing the target gene.
03
Purify the PCR product using a DNA purification kit.
04
Submit the purified DNA fragment for sequencing or use it for mutagenesis techniques such as site-directed mutagenesis.
05
Analyze the sequencing results or verify the mutated gene sequence using appropriate methods.

Who needs sequencing and mutagenesis of?

01
Researchers studying genetic mutations or variations in DNA sequences.
02
Scientists working on genetic engineering projects.
03
Biotechnologists involved in gene editing or genome editing techniques.
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Sequencing and mutagenesis of refer to the process of determining the order of nucleotides in a DNA or RNA molecule and inducing changes in the genetic material.
Researchers, scientists, or institutions conducting genetic studies are required to file sequencing and mutagenesis reports.
Sequencing and mutagenesis reports can be filled out online through a designated platform or submitted via email/mail according to the specific requirements.
The purpose of sequencing and mutagenesis is to understand genetic information, study gene functions, and potentially create genetic modifications for research or medical purposes.
The report must include details of the genetic sequence being studied, the techniques used, any mutations induced, and the implications of the findings.
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