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Get the free IHC & Special Stains Menu Clinical and Specimen Information ...

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Children's Hospital Los Angeles Alexander R. Judging, MD Department of Pathology & Laboratory Medicine PathologistinChief and Laboratory Director Phone: 323.361.2469, 323.361.2426 Fax: 323.361.8004
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01
Prepare the tissue sample by fixing it in formalin and embedding it in paraffin wax.
02
Cut thin sections of the tissue sample and mount them on slides.
03
Deparaffinize and rehydrate the tissue sections.
04
Block endogenous peroxidase activity with hydrogen peroxide.
05
Incubate the tissue sections with primary antibody specific to the protein of interest.
06
Wash the tissue sections to remove unbound primary antibody.
07
Apply secondary antibody labeled with an enzyme, such as horseradish peroxidase.
08
Develop the color reaction using a chromogen substrate.
09
Counterstain the tissue sections if necessary.
10
Mount coverslips on the slides and examine under a microscope.

Who needs ihc ampamp special stains?

01
Pathologists and medical laboratory professionals who are investigating the presence and distribution of specific proteins in tissue samples.
02
Researchers in the fields of biology, medicine, and biotechnology who are studying the expression of proteins in cells and tissues.
03
Medical professionals diagnosing diseases such as cancer, autoimmune disorders, and infectious diseases that require detailed examination of tissue samples.
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IHC (Immunohistochemistry) and special stains are laboratory techniques used to identify specific proteins and microorganisms in tissue samples, aiding in the diagnosis of various diseases.
Typically, healthcare providers and laboratories that perform IHC and special stains on tissue samples are required to document and file these results.
To fill out IHC and special stains documentation, one should provide patient information, specimen details, staining methods used, and the interpretation of results.
The purpose of IHC and special stains is to enhance the visualization of specific cellular components, helping pathologists to diagnose diseases and determine treatment options.
Information to be reported includes patient demographic information, specimen identification, staining methodology, results of the staining, and conclusions drawn from the interpretations.
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