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Children's Hospital Los Angeles Alexander R. Judging, MD Department of Pathology & Laboratory Medicine PathologistinChief and Laboratory Director Phone: 323.361.2469, 323.361.2426 Fax: 323.361.8004

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How to fill out immunohistochemistry special stains

01

Prepare the tissue slides by deparaffinizing and rehydrating them.

02

Block any endogenous peroxidase activity to prevent nonspecific staining.

03

Apply the primary antibody specific to the antigen of interest and incubate.

04

Wash off any unbound primary antibody.

05

Apply a secondary antibody conjugated to a detection system (e.g. HRP) to bind to the primary antibody.

06

Wash off any unbound secondary antibody.

07

Apply the chromogen substrate to produce a visible signal at the site of antigen-antibody binding.

08

Counterstain the slides if desired, dehydrate, and mount for analysis.

Who needs immunohistochemistry special stains?

01

Pathologists who need to identify specific proteins or antigens in tissue samples for diagnostic purposes.

02

Researchers studying the expression of particular markers in biological samples.

03

Veterinary pathologists examining animal tissues for disease markers or research purposes.

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What is immunohistochemistry special stains?

Immunohistochemistry special stains are laboratory techniques used to detect specific proteins in tissue samples.

Who is required to file immunohistochemistry special stains?

Pathologists and laboratory technicians are required to file immunohistochemistry special stains.

How to fill out immunohistochemistry special stains?

Immunohistochemistry special stains are filled out by documenting the specific proteins detected in the tissue samples.

What is the purpose of immunohistochemistry special stains?

The purpose of immunohistochemistry special stains is to help diagnose diseases and determine treatment options based on protein expression.

What information must be reported on immunohistochemistry special stains?

The information reported on immunohistochemistry special stains includes the specific proteins detected, their location in the tissue, and the staining intensity.

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