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EXERCISE 8A Lab Procedures Important: Save all of your leftover samplessupernatant, pellet, clarified whey, column chromatography fractions, etc. until the end of the lab period. Make a habit of placing
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01
Prepare the size exclusion chromatography column by washing it with appropriate buffer.
02
Prepare your sample by ensuring it is in the same buffer as the column.
03
Load the sample onto the column and start the chromatography run.
04
Collect fractions based on the elution profile of the sample.
05
Analyze the fractions to determine the size of the different components in your sample.

Who needs size exclusion chromatography instruction?

01
Scientists and researchers working in biochemistry, biotechnology, and pharmaceutical industries who need to separate and analyze biomolecules based on their size.
02
Students and lab technicians learning about chromatography techniques in academic settings.
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Size exclusion chromatography instruction is a technique used for separating molecules based on their size.
Researchers and scientists conducting experiments or studies involving size exclusion chromatography are required to file the instruction.
The instruction should be filled out with details of the chromatography method, equipment used, samples analyzed, and any relevant data obtained.
The purpose is to document and report the specifics of size exclusion chromatography experiments for reference and future replication.
Information such as method used, equipment details, sample types, and data analysis should be reported.
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