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University of Alabama in HuntsvilleLOUIS Honors Capstone Projects and ThesesHonors College4292019NMR Analysis of Metabolomic Changes in Levels of Neurotransmitters in Rodents Sana Salman KhadairFollow
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01
Prepare the samples by extracting the metabolites using an appropriate method.
02
Dilute the sample with a deuterated solvent to provide a lock signal.
03
Transfer the sample into an NMR tube and load it into the NMR spectrometer.
04
Acquire the NMR spectrum using the appropriate parameters and pulse sequences.
05
Process the NMR data using software to phase correction, baseline correction, and peak picking.
06
Assign peaks to specific metabolites by comparing with databases or standards.
07
Quantify the metabolites by integrating peak areas and comparing with internal or external standards.
08
Interpret the results and analyze the metabolic profile of the sample.

Who needs nmr analysis of metabolomic?

01
Researchers studying biological systems and metabolism
02
Pharmaceutical companies developing new drugs
03
Agricultural scientists working on crop improvement
04
Doctors and clinicians investigating diseases and metabolic disorders
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NMR analysis of metabolomic is a technique used to study the metabolic profile of biological samples by analyzing their nuclear magnetic resonance spectra.
Researchers and scientists conducting metabolomic studies are required to file nmr analysis of metabolomic.
To fill out nmr analysis of metabolomic, one needs to conduct the NMR analysis on the biological samples and then report the results and findings.
The purpose of nmr analysis of metabolomic is to understand the metabolic pathways and changes in metabolite levels in biological samples.
The information reported on nmr analysis of metabolomic includes the metabolite identification, quantification, and any relevant findings.
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