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On Miriam×P a g e s 1 1 7 6 1 1 1 8 1 0t BJ\'/*SCRIPT | I MANET FEDERAL V O LU M E 2 6ESISTER 1934^^^ / an n t e d IN U M B ER236W Washington, Friday, December 8, 1961C o n ten ts A g r i cu l t
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How to fill out wt-pe prime editing with

01
Prepare the reaction mix by combining the DNA template with the prime editing components such as the Cas9 nickase, reverse transcriptase, and prime editing guide RNA.
02
Incubate the reaction mix at the appropriate temperature for prime editing to occur.
03
Purify the edited DNA using standard molecular biology techniques.
04
Analyze the edited DNA for the desired mutations or modifications.

Who needs wt-pe prime editing with?

01
Researchers looking to make precise and specific edits to DNA sequences without introducing double-stranded breaks.
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wt-pe prime editing is used with a Cas9 variant fused to a reverse transcriptase enzyme.
Researchers and scientists who are conducting gene editing experiments using wt-pe prime editing are required to file.
wt-pe prime editing forms can be filled out electronically or on paper, following the instructions provided by the regulatory agency.
wt-pe prime editing is used to make precise changes to DNA sequences for research purposes, such as correcting genetic mutations or studying gene function.
Information such as the target gene, editing sequence, experimental protocol, and expected outcomes must be reported on wt-pe prime editing forms.
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