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Maulana Abdul Adam Azad University of Technology, West Bengal Ward No.08, Post Simchat, PS Haringhata, Nadia 741249INVITATION FOR QUOTATION Package Code: TEQIPIII/2019/WB/makeup/166 Package Name:

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How to fill out mini trans- blot module

01

Prepare the gel by pouring the appropriate percentage of agarose gel into the gel tray.

02

Add the DNA samples into the wells using a micropipette.

03

Run the gel at a specific voltage for a certain amount of time to separate the DNA fragments.

04

After the electrophoresis is complete, carefully remove the gel from the tray and place it on the mini trans-blot module.

05

Assemble the transfer sandwich with the gel, filter paper, membrane, and cassette according to the manufacturer's instructions.

06

Fill the transfer buffer into the mini trans-blot module reservoirs and connect the electrodes.

07

Run the transfer at a specific voltage for a certain amount of time to transfer the DNA onto the membrane.

08

Disassemble the transfer sandwich, remove the membrane, and proceed with hybridization or detection steps.

Who needs mini trans- blot module?

01

Researchers in molecular biology labs who need to transfer DNA fragments from agarose gels onto membranes for further analysis.

02

Scientists studying gene expression, genomics, or genetic variation who need to perform Southern blotting or other nucleic acid hybridization techniques.

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What is mini trans- blot module?

Mini trans-blot module is a small module used for transferring proteins from a gel to a membrane in protein analysis.

Who is required to file mini trans- blot module?

Researchers and scientists conducting protein analysis experiments may be required to file mini trans-blot module.

How to fill out mini trans- blot module?

Mini trans-blot module can be filled out by providing information about the proteins being transferred, experimental conditions, and any relevant findings.

What is the purpose of mini trans- blot module?

The purpose of mini trans-blot module is to document the transfer of proteins in protein analysis experiments.

What information must be reported on mini trans- blot module?

Information such as protein type, experimental setup, transfer conditions, and any observed results must be reported on mini trans-blot module.

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