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Aura mine AFB Stain SOP Author: Anne Morris Document Number: Pro67C13Effective (or Post) Date: 10 Feb 09 Document Origin; Site Laboratory Company: BiotechnologySMILE Approved by: Peggy Coulter Review
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How to fill out auramine stain sop

01
Prepare the auramine stain reagent by mixing the auramine O powder with distilled water according to the manufacturer's instructions.
02
Fix the specimen on a slide using heat fixation or other suitable method.
03
Flood the fixed specimen with the auramine stain reagent and incubate for the recommended time.
04
Rinse the slide with distilled water to remove excess stain.
05
Counterstain with a suitable counterstain like potassium permanganate or auramine-rhodamine.
06
Rinse the slide again and allow it to air dry before examining under a fluorescence microscope.

Who needs auramine stain sop?

01
Laboratory technicians and professionals working in microbiology, pathology, or related fields who need to stain and examine specimens for acid-fast bacteria like Mycobacterium tuberculosis.

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Auramine stain sop is a standard operating procedure used in microbiology laboratories for staining acid-fast bacteria.
Laboratory technicians and scientists working in microbiology laboratories are required to follow and file auramine stain sop.
Auramine stain sop should be filled out by following the step-by-step instructions provided in the document, including proper staining techniques and safety precautions.
The purpose of auramine stain sop is to ensure consistent and accurate staining of acid-fast bacteria for proper identification and diagnosis.
Auramine stain sop must include information on reagents used, staining procedure, safety measures, and recording of results.
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