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Test Definition: HIP Historic Acid, Urine___ OverviewMethod Name Calorimetry © High Performance Liquid Chromatography/Tandem Mass Spectrometry (LCS/MS) NY State Available YesSpecimenSpecimen Type
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Prepare the HPLC instrument by setting up the column, mobile phase, and detector.
02
Inject the sample into the instrument.
03
Start the chromatography run and monitor the separation of compounds.
04
Analyze the data generated by the detector to determine the retention times and peak shapes of the compounds.
05
Interpret the chromatogram to identify and quantify the compounds in the sample.

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Analytical chemists who need to separate and analyze complex mixtures of compounds.
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Scientists working in pharmaceutical, environmental, and food industries for quality control and research purposes.
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Researchers studying biomolecules such as proteins, peptides, and nucleic acids.
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High-performance liquid chromatography (HPLC) is a technique used to separate, identify, and quantify components in a mixture.
Researchers, scientists, and professionals working in analytical laboratories are usually required to file a high-performance liquid chromatography.
To fill out a high-performance liquid chromatography, one needs to input information about the sample being analyzed, the type of column and solvent used, and the conditions of the HPLC run.
The purpose of a high-performance liquid chromatography is to separate, identify, and quantify components in a mixture based on their interactions with the mobile and stationary phases.
The information reported on a high-performance liquid chromatography typically includes peak retention times, peak areas, sample concentrations, and calibration curve data.
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