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Results of polo Muteness Lab Exercise pM: Session Four Name ___ Date ___ Section ___ Name of partner ___ Paste your gel photograph below and explain the bands that you observed. Notice the sizes of your
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01
Prepare a template DNA containing the desired mutation.
02
Transform the template DNA into competent bacteria using a method such as heat shock or electroporation.
03
Plate the transformed bacteria on selective agar containing antibiotics to ensure only bacteria with the desired mutation survive.
04
Incubate the plates overnight at the appropriate temperature.
05
Examine the plates for successful transformation based on the presence of fluorescence from the pglo gene.

Who needs results of pglo mutagenesis?

01
Researchers studying gene expression and regulation may need the results of pglo mutagenesis to investigate the effects of specific mutations on protein function.
02
Biotechnologists who are developing genetically modified organisms (GMOs) for various applications may also require the results of pglo mutagenesis to verify successful gene editing.
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The results of pglo mutagenesis include information on genetic changes induced in the pglo gene.
Researchers or institutions conducting the pglo mutagenesis experiment are required to file the results.
The results of pglo mutagenesis can be filled out by documenting the specific genetic changes observed, experimental methods used, and any relevant data.
The purpose of the results of pglo mutagenesis is to document the outcomes of the genetic modification experiment for research and regulatory purposes.
The results of pglo mutagenesis must report details of the genetic changes induced, methods used, any observed phenotypic effects, and other relevant data.
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