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Test Method Protocol for the NHK Neutral Red Uptake Cytotoxicity Assay
Phase III Validation Study:
November 4, 2003TEST METHOD PROTOCOL for the NHK Neutral Red Uptake Cytotoxicity Test for Basal Cytotoxicity
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How to fill out neutral red uptake for
How to fill out neutral red uptake for
01
Prepare a stock solution of neutral red dye in water.
02
Plate the cells to be tested in a 96-well plate.
03
Add the neutral red stock solution to each well containing cells.
04
Incubate the plate for a specified amount of time to allow the dye to be taken up by the cells.
05
Remove the dye and wash the cells to remove excess dye.
06
Measure the absorbance of the dye taken up by the cells using a spectrophotometer.
Who needs neutral red uptake for?
01
Researchers studying cell viability and cytotoxicity.
02
Pharmaceutical companies testing the effects of drugs on cells.
03
Scientists investigating the mechanism of action of compounds on cells.
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What is neutral red uptake for?
Neutral red uptake is a common assay used to measure cell viability and cytotoxicity by staining live cells red.
Who is required to file neutral red uptake for?
Researchers and scientists conducting cell viability studies are required to perform and file neutral red uptake assays.
How to fill out neutral red uptake for?
To fill out a neutral red uptake assay, cells are incubated with the dye, washed, lysed, and the dye is extracted and measured using a spectrophotometer.
What is the purpose of neutral red uptake for?
The purpose of neutral red uptake is to assess cell viability and determine the effects of various treatments or conditions on cell health.
What information must be reported on neutral red uptake for?
The results of the assay, including absorbance values, cell viability percentages, and any relevant experimental conditions, must be reported on a neutral red uptake form.
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