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This document serves as a service request form for next-generation sequencing (NGS) library preparation, outlining user information and sample details required for the Functional Genomics Laboratory.
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How to fill out NGS library prep Service Request

01
Start by accessing the NGS library prep Service Request form online.
02
Fill in your contact information, including your name, email, and phone number.
03
Specify the type of NGS library prep service you require.
04
Indicate the sample type and quantity you will be submitting.
05
Provide any special instructions or requirements for your library prep.
06
Review all information for accuracy.
07
Submit the completed form.
08
Save a copy of the confirmation for your records.

Who needs NGS library prep Service Request?

01
Researchers conducting genomic studies.
02
Laboratories offering sequencing services.
03
Anyone requiring library preparation for NGS analysis.
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NGS library prep takes time and effort Manual library preparation takes between 4 and 8 hours of work. Typically, a full day is required for preparation of libraries for DNA and two days for mRNA sequencing libraries. With long, multi-step workflows, there is always ample opportunity for human error.
NGS Library Prep Fees per Sample LibraryKitCampus Illumina mRNA-Seq Stranded mRNA Prep, Ligation $137 Illumina Total RNA-seq Stranded Total RNA Prep, Ligation $182 KAPA DNA Seq KAPA HyperPrep (WGS, Other) $129 Agilent Exome Capture, 1-16 samples Sure Select, v5 or v6+UTR baits $3554 more rows
The Illumina DNA Prep kit uses tagmentation technology for the construction of genomic DNA sequencing libraries with an average insert size of approximately 300–600 bp and minimal pcr amplification. The recommended input for library construction is 10–200 ng of DNA which should be delivered in a volume of 15–30 ul.
NGS library prep takes time and effort Manual library preparation takes between 4 and 8 hours of work. Typically, a full day is required for preparation of libraries for DNA and two days for mRNA sequencing libraries. With long, multi-step workflows, there is always ample opportunity for human error.
Library preparation is the first step of next generation sequencing. It allows DNA or cDNA to adhere to the sequencing flow cell and allows the sample to be identified. Two common methods of library preparation are ligation-based library prep and tagmentation-based library prep.

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NGS library prep Service Request is a formal request process for the preparation of DNA or RNA libraries for next-generation sequencing.
Researchers, laboratories, and institutions that intend to conduct sequencing using next-generation sequencing technology are required to file this request.
To fill out the NGS library prep Service Request, provide the necessary details such as project title, sample information, specific service requirements, and contact information, ensuring all fields are completed accurately.
The purpose of the NGS library prep Service Request is to streamline the process of preparing sequencing libraries, ensuring all necessary information is collected for efficient and accurate processing.
The information that must be reported includes the sample type, quantity, desired sequencing technology, expected turnaround time, and contact information for the requester.
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