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Product Information
Product Name
AliasH9 Crop
WA09(Long)Lot Number
Parent Material
Depositor
Banked by
Thaw RecommendationWB20971
WA09(Long)MCB01
University of Wisconsin
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How to fill out cre recombination mediated cassette
How to fill out cre recombination mediated cassette
01
Design cre recombination mediated cassette with loxP sites flanking the DNA sequence to be excised.
02
Clone the cassette into a plasmid vector using standard molecular biology techniques.
03
Transfect the plasmid into cells expressing Cre recombinase enzyme.
04
Allow Cre recombinase to bind to the loxP sites and catalyze recombination, resulting in excision of the DNA sequence.
Who needs cre recombination mediated cassette?
01
Researchers studying gene function and regulation
02
Scientists interested in genetic manipulation and gene editing techniques
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What is cre recombination mediated cassette?
Cre recombinase-mediated cassette exchange (RMCE) is a technology that allows for the site-specific integration of DNA sequences into a target genome.
Who is required to file cre recombination mediated cassette?
Researchers or scientists working on genetic modification projects may be required to file cre recombination mediated cassette.
How to fill out cre recombination mediated cassette?
To fill out cre recombination mediated cassette, one must provide detailed information about the DNA sequences to be integrated, the target genome, and the methods used for integration.
What is the purpose of cre recombination mediated cassette?
The purpose of cre recombination mediated cassette is to enable precise genetic modifications in research experiments.
What information must be reported on cre recombination mediated cassette?
The information to be reported on cre recombination mediated cassette includes details about the DNA sequences, target genome, integration methods, and any potential risks or side effects.
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