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01
Prepare protein samples from different conditions or treatments.
02
Digest the proteins into peptides using a protease enzyme.
03
Label the peptides from each condition with different itraq reagents.
04
Combine the itraq-labeled peptides into a single sample for mass spectrometry analysis.
05
Run the mass spectrometry experiment to quantify the abundance of peptides from each condition.
06
Analyze the data to determine the relative abundance of proteins across different conditions.

Who needs itraq-based quantitative proteomics of?

01
Researchers studying changes in protein expression levels in response to different conditions or treatments.
02
Scientists interested in identifying potential biomarkers or drug targets.
03
Proteomics labs looking to quantify protein expression levels in a high-throughput manner.
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It is a method used to quantify proteins in a biological sample.
Researchers and scientists conducting proteomics studies.
By following standardized protocols and analyzing the data obtained.
To compare protein expression levels between different samples.
Quantitative data on protein abundance in the samples.
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