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UNIVERSITTSKLINIKUM HAMBURGEPPENDORFInstitut for ImmunologieProf. Dr. Marcus AltfeldOptimization of nobodies for in vivo targeting of P2X7 ion channel on brain microglia and kidney T cellsDissertation our
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01
Select a diverse library of nanobodies.
02
Screen the library for high binding affinity and specificity.
03
Use structure-based design to engineer mutations for improved stability and solubility.
04
Optimize conditions for expression and purification of the selected nanobodies.
05
Characterize the optimized nanobodies for their binding properties and stability.

Who needs optimization of nanobodies for?

01
Researchers working in the field of antibody therapeutics
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Biotechnology companies developing diagnostic assays
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Scientists studying protein-protein interactions
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The optimization of nanobodies is for enhancing the properties and functions of nanobodies for various applications in research, diagnostics, and therapeutics.
Researchers, scientists, and biotech companies who are working on developing or using nanobodies may be required to file optimization reports.
The optimization of nanobodies can be filled out by providing detailed information on the methodology, results, and conclusions of the optimization process.
The purpose of optimization of nanobodies is to improve their stability, affinity, and specificity for their intended applications.
Information such as the design strategy, experimental procedures, analytical data, and characterization of the optimized nanobodies must be reported.
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