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Cell Line Name
Well Lot Number
Provider
Banked By
Thaw and Culture
Recommendations
Culture PlatformThaw and Culture DetailsSTAN011i1231
DB31129
Stanford University Laboratory of Dr. Marlene Rabinovitz
Stanford
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How to fill out wicell recommends thawing using
How to fill out wicell recommends thawing using
01
Remove vial from liquid nitrogen and immediately thaw in a 37°C water bath until only a small ice crystal remains in the vial.
02
Swirl the vial gently to mix the remaining ice with the cells.
03
Immediately transfer the contents of the vial to a sterile tube containing pre-warmed culture medium.
04
Gently mix by pipetting up and down several times to ensure even distribution of cells.
Who needs wicell recommends thawing using?
01
Researchers and scientists who work with stem cell lines from WiCell would benefit from following the recommended thawing procedure.
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What is wicell recommends thawing using?
WiCell recommends thawing using a 37°C water bath.
Who is required to file wicell recommends thawing using?
Researchers and lab technicians working with stem cells are required to follow WiCell's recommendations for thawing.
How to fill out wicell recommends thawing using?
To fill out WiCell's recommendations for thawing, carefully follow the instructions provided by WiCell for using a 37°C water bath.
What is the purpose of wicell recommends thawing using?
The purpose of following WiCell's recommendations for thawing is to ensure successful recovery and viability of stem cells after freezing.
What information must be reported on wicell recommends thawing using?
The information that must be reported includes the date and time of thawing, the number of vials thawed, and any observations during the thawing process.
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