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Certificate of Analysis Thaw and Culture Details Cell Line Name Well Lot Number Parent Material Provider/Client Banked By Thaw and Culture Recommendations ProtocolWA09 WB67843 WA09WB0090 University

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How to fill out wicell recommends thawing 1

01

Thaw the WiCell recommends thawing 1 vial at room temperature by gently swirling it in a water bath set at 37°C.

02

Once completely thawed, transfer the contents to a sterile tube and centrifuge at low speed to collect the cells.

03

Remove the supernatant and resuspend the cells in the desired medium for further experiments.

04

Count the cells using a hemocytometer or automated cell counter to determine cell concentration before use.

Who needs wicell recommends thawing 1?

01

Researchers working with cell cultures that have been frozen using the WiCell recommends thawing 1 protocol.

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What is wicell recommends thawing 1?

Wicell recommends thawing 1 is a guideline for the thawing process of stem cells.

Who is required to file wicell recommends thawing 1?

Medical professionals and researchers working with stem cells are required to follow wicell recommends thawing 1.

How to fill out wicell recommends thawing 1?

Wicell recommends thawing 1 should be filled out following the provided instructions and guidelines.

What is the purpose of wicell recommends thawing 1?

The purpose of wicell recommends thawing 1 is to ensure the proper thawing procedure for stem cells.

What information must be reported on wicell recommends thawing 1?

Information such as date of thawing, thawing temperature, duration, and any observations must be reported on wicell recommends thawing 1.

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