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Certificate of Analysis Thaw and Culture Details Cell Line Name Well Lot Number Parent Material Provider/Client Banked By Thaw and Culture Recommendations Protocols(IMR90)4 WB67846 IPS(IMR90)4WB65317
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How to fill out mtesrplus and matrigel
How to fill out mtesrplus and matrigel
01
Thaw the Matrigel on ice overnight.
02
Dilute the Matrigel with cold media to desired concentration.
03
Mix the Matrigel solution gently to avoid bubbles.
04
Plate the Matrigel solution in the desired tissue culture dishes.
05
Allow the Matrigel to solidify at 37°C for 30 minutes before adding cells.
06
Prepare the mtesrplus solution as per manufacturer's instructions.
07
Add the mtesrplus solution to the cells cultured on the Matrigel.
Who needs mtesrplus and matrigel?
01
Researchers working in the field of stem cell culture and maintenance
02
Scientists studying cell differentiation and organoid formation
03
Laboratories conducting drug screening assays on 3D cell cultures
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What is mtesrplus and matrigel?
mtesrplus is a serum-free, well-defined maintenance medium that promotes the growth and expansion of human pluripotent stem cells. Matrigel is a gelatinous protein mixture used in cell culture that serves as a scaffold for supporting cell growth.
Who is required to file mtesrplus and matrigel?
Research institutions, biotechnology companies, and laboratories working with human pluripotent stem cells may be required to use mtesrplus and matrigel in their experiments.
How to fill out mtesrplus and matrigel?
To fill out mtesrplus and matrigel, follow the instructions provided by the manufacturer for proper preparation and usage.
What is the purpose of mtesrplus and matrigel?
The purpose of mtesrplus and matrigel is to create optimal conditions for the growth, maintenance, and expansion of human pluripotent stem cells in culture.
What information must be reported on mtesrplus and matrigel?
Information such as batch number, expiration date, storage conditions, and any deviations from standard protocols must be reported when using mtesrplus and matrigel.
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