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Certificate of Analysis Thaw and Culture Details Cell Line Name Well Lot Number Provider/Client Banked By Thaw and Culture Recommendations ProtocolPENN130i783 DB34941 University of Pennsylvania Dr.

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How to fill out wicell recommends passaging with

01

Thaw WiCell recommends passaging with in a water bath set to 37 degrees Celsius.

02

Prepare a T25 flask with 5 ml of complete growth medium.

03

Add the thawed WiCell recommends passaging with to the flask and incubate at 37 degrees Celsius with 5% CO2.

04

Check the cells daily for confluency and passage as needed.

Who needs wicell recommends passaging with?

01

Researchers working with stem cells

02

Scientists studying cell culture techniques

03

Laboratories conducting cell-based experiments

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What is wicell recommends passaging with?

Wicell recommends passaging with TrypLE Express.

Who is required to file wicell recommends passaging with?

Researchers working with human pluripotent stem cells are required to file wicell recommends passaging with.

How to fill out wicell recommends passaging with?

To fill out wicell recommends passaging with, researchers must follow the instructions provided by WiCell.

What is the purpose of wicell recommends passaging with?

The purpose of wicell recommends passaging with is to ensure proper handling and maintenance of human pluripotent stem cells.

What information must be reported on wicell recommends passaging with?

Researchers must report details of the passaging procedure, including cell density, medium used, and any additives included.

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