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Thaw and Culture DetailsCell Line Name Well Lot Number Provider Banked By Thaw and Culture Recommendations Culture PlatformPENN135i308 DB34725 University of Pennsylvania Dr. Daniel Radar Penn Institute

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How to fill out wicell feeder dependent protocol

01

Thaw the Wicell feeder cells according to the manufacturer's instructions.

02

Plate the feeder cells in a gelatin-coated dish and culture them in feeder cell medium until they reach confluency.

03

Aspirate the feeder cell medium and wash the cells with PBS.

04

Add the desired pluripotent stem cell culture media on top of the feeder cells.

Who needs wicell feeder dependent protocol?

01

Researchers working with pluripotent stem cells that require feeder cells for maintenance and growth.

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What is wicell feeder dependent protocol?

WiCell Feeder Dependent Protocol is a protocol used for culturing stem cells that require feeder cells for growth and maintenance.

Who is required to file wicell feeder dependent protocol?

Researchers and laboratories working with feeder-dependent stem cells are required to file WiCell Feeder Dependent Protocol.

How to fill out wicell feeder dependent protocol?

WiCell Feeder Dependent Protocol can be filled out by providing detailed information on the culture conditions, media components, and maintenance procedures for feeder-dependent stem cells.

What is the purpose of wicell feeder dependent protocol?

The purpose of WiCell Feeder Dependent Protocol is to ensure consistency and proper documentation of the culture methods used for feeder-dependent stem cells.

What information must be reported on wicell feeder dependent protocol?

Information such as cell line name, passage number, culture medium composition, seeding density, and subculture frequency must be reported on WiCell Feeder Dependent Protocol.

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