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ADAward Number: W81XWH0410630TITLE: Surface Enhanced Raman Spectroscopy for Monitoring Lactate and GlucosePRINCIPAL INVESTIGATOR: Matthew R. Glucksberg, Ph.D. Joseph Walsh, Ph.D. Richard Van Duyne,
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To fill out surface enhanced Raman spectroscopy, follow these steps:
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Prepare a clean and smooth surface, such as a glass substrate.
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Prepare a suitable metal substrate, such as gold or silver.
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Incubate the metal substrate in a solution containing the target molecules you want to analyze.
05
Rinse the metal substrate with a suitable solvent to remove any impurities.
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Dry the metal substrate carefully to ensure a uniform surface.
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Use a pipette to drop a small amount of the prepared metal substrate onto the clean surface.
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Spread the metal substrate evenly over the entire surface using a suitable tool, such as a spin coater.
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Allow the metal substrate to dry completely before performing Raman spectroscopy.
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Once the substrate is dry, you can analyze it using a Raman spectrometer with appropriate excitation wavelength.
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Record the Raman spectra and analyze the enhancement effect provided by the metal substrate.

Who needs surface enhanced raman spectroscopy?

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Surface enhanced Raman spectroscopy is of interest to various research fields and industries including:
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- Biomedical researchers studying biomolecules, proteins, and DNA.
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- Environmental scientists studying pollutants and contaminants.
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- Forensic scientists analyzing trace evidence and chemical components.
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- And many more who require sensitive and specific molecular analysis capabilities.
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Surface enhanced Raman spectroscopy (SERS) is a sensitive analytical technique that enhances the Raman scattering signal of molecules adsorbed on rough metal surfaces or nanoparticles, allowing for the detection of low concentrations of substances.
Typically, researchers and institutions involved in studies requiring Raman spectroscopy data may file for surface enhanced Raman spectroscopy, particularly when reporting findings in scientific publications or compliance with regulatory requirements.
Filling out surface enhanced Raman spectroscopy typically involves documenting the experimental setup, sample preparation, SERS substrate used, the laser parameters, and the spectral data obtained during the analysis.
The purpose of surface enhanced Raman spectroscopy is to provide enhanced sensitivity and specificity for molecular detection, enabling the analysis of small quantities of substances for applications in chemistry, biology, and medicine.
Information that must be reported includes sample details, the methodology used (including the SERS substrate), spectral data, any modifications in protocol, and relevant findings that arise from the analysis.
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