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EWERS, European Journal of Engineering Research and Science Vol. 5, No. 11, November 2020Characterization of MultiDrug Resistant GramNegative Bacteria Present in Fresh Leafy & Salad Vegetables in Dhaka,
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01
Collect the isolates or samples that need to be characterized for multi-drug resistance.
02
Prepare the necessary reagents and media for conducting the characterization.
03
Perform antimicrobial susceptibility testing using standard methods, such as the disk diffusion or broth microdilution method.
04
Determine the minimum inhibitory concentration (MIC) of different antibiotics against the isolates.
05
Interpret the susceptibility results based on established breakpoints and guidelines.
06
Determine the presence or absence of specific resistance genes or mutations using molecular techniques, such as polymerase chain reaction (PCR) or whole-genome sequencing.
07
Analyze and interpret the molecular data to identify the multi-drug resistance profile of the isolates.
08
Characterize the isolates based on their resistance patterns, genetic mechanisms of resistance, and associated factors, such as plasmids or mobile genetic elements.
09
Document the findings in a comprehensive report, including the key characteristics and properties of the multi-drug resistant strains.
10
Use the characterization results to guide treatment strategies, infection control measures, and surveillance efforts.
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Continuously update and refine the characterization methods and techniques to stay aligned with emerging resistance patterns and developments in the field.

Who needs characterization of multi-drug resistant?

01
Healthcare professionals involved in diagnosing and treating bacterial infections
02
Microbiologists and laboratory personnel performing antimicrobial susceptibility testing
03
Pharmaceutical companies researching and developing new antimicrobial agents
04
Public health agencies and surveillance programs monitoring the prevalence and spread of multi-drug resistant organisms
05
Epidemiologists studying the factors contributing to antimicrobial resistance
06
Infection control teams working to prevent and control the spread of multi-drug resistant bacteria
07
Researchers investigating the mechanisms and evolution of antibiotic resistance
08
Veterinarians and livestock producers concerned with antibiotic use in animals and its impact on resistance
09
Policy makers and regulatory bodies making decisions related to antimicrobial use and resistance
10
Patients with suspected or confirmed multi-drug resistant infections and their healthcare providers
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Characterization of multi-drug resistant refers to the process of identifying and describing organisms that exhibit resistance to multiple antimicrobial agents, which can complicate treatment options.
Healthcare facilities, laboratories, and public health organizations that identify multi-drug resistant organisms are required to file characterization.
To fill out characterization of multi-drug resistant, one must provide detailed information on the organism's characteristics, resistance patterns, clinical significance, and any relevant epidemiological data.
The purpose of characterization of multi-drug resistant is to monitor resistance trends, inform treatment decisions, and aid public health interventions to control the spread of resistant infections.
Information that must be reported includes the organism's species, resistance genes, susceptibility testing results, sources of infection, and patient demographics.
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