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___Masarykova univerzita vBrn? Lka?sk fakultaBakal?sk prce2008 Lucie Lyskov ___Masarykova univerzita v Brn? Lka?sk fakultaRealtime PCR a jeho vyu?it v klinick molekulrn diagnosticeBakal?sk prce v
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How to fill out real - time pcr

01
Prepare the real-time PCR reaction mix by combining the necessary reagents such as DNA or RNA template, primers, probe, nucleotides, buffer, and enzyme.
02
Set up the thermal cycling conditions on the real-time PCR instrument according to the specific protocols or requirements.
03
Pipette the reaction mix into the wells of a PCR plate or tubes.
04
Place the plate or tubes in the real-time PCR instrument.
05
Start the real-time PCR run and closely monitor the amplification results in real-time.
06
Analyze the amplification curves or data generated by the real-time PCR instrument to determine the presence or quantity of the target DNA or RNA.
07
Interpret the results based on the experimental design and objectives.

Who needs real - time pcr?

01
Real-time PCR is widely used in various fields such as molecular biology, genetics, medical diagnostics, and research.
02
It is commonly utilized by scientists, researchers, clinical laboratories, and healthcare professionals.
03
Specific applications include gene expression analysis, pathogen detection, genotyping, viral load determination, and genetic testing.
04
Real-time PCR is also valuable in drug discovery, forensic analysis, agriculture, and environmental monitoring.
05
Anyone who requires accurate and sensitive detection or quantification of nucleic acids can benefit from real-time PCR.

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Real-time PCR, also known as quantitative PCR (qPCR), is a laboratory technique used to amplify and simultaneously quantify a targeted DNA molecule. It allows for the monitoring of the PCR process in real time.
Individuals or entities involved in certain regulatory or compliance activities, particularly related to the pharmaceutical, biotechnology, and environmental sectors, are typically required to file real-time PCR.
Filling out a real-time PCR involves providing accurate data regarding the PCR process, including sample identification, reaction conditions, target sequence information, and the quantification results.
The purpose of real-time PCR is to detect and quantify nucleic acids in a sample, facilitating research, diagnostics, and monitoring purposes in various fields, including medical diagnostics, genetics, and forensics.
The information that must be reported includes sample ID, target gene details, amplification data, threshold cycle (Ct) values, and quantitative results based on standard curves.
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