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OVEREENKOMST IN TOEPASSING VAN ART 56, 1 ter financiering van een pilootstudie betreffende de gecontroleerde introductie van NextGenerationSequencing in routine diagnostiek in oncologie en hematooncologie Gelet
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How to fill out next generation sequencing in

01
Collect the DNA or RNA sample that you want to sequence.
02
Extract the DNA or RNA from the collected sample.
03
Prepare the library of DNA or RNA fragments by fragmenting the sample and adding adapters.
04
Amplify the library using PCR to increase the amount of DNA or RNA fragments.
05
Perform quality control checks to ensure the library is suitable for sequencing.
06
Load the library onto a sequencing platform, such as an Illumina sequencer.
07
Run the sequencing process, which involves the incorporation of fluorescently-labeled nucleotides and detection of emitted light signals.
08
Analyze the raw sequencing data to obtain the DNA or RNA sequence information.
09
Perform data analysis and interpretation to identify genetic variants, gene expression levels, or other relevant information.
10
Validate the findings through additional experiments or comparison with existing knowledge.
11
Publish or report the results of the next generation sequencing study.

Who needs next generation sequencing in?

01
Researchers studying genetics, genomics, or molecular biology to investigate the genetic basis of diseases, traits, or biological processes.
02
Clinical laboratories and healthcare professionals to diagnose genetic disorders or detect mutations that may impact patient care.
03
Pharmaceutical companies to identify potential drug targets or understand drug interactions at a molecular level.
04
Agricultural organizations to improve crop production and breeding by analyzing the genome of plants and animals.
05
Forensic laboratories to analyze DNA evidence and aid in criminal investigations.
06
Conservationists and ecologists to study biodiversity and the genetic health of endangered species.
07
Bioinformatics experts to develop and improve algorithms and software tools for analyzing next generation sequencing data.
08
Academic institutions and educational organizations to teach and train future scientists in the field of genomics.
09
Government agencies and policymakers to make informed decisions related to public health, food safety, and environmental management based on genomic data.
10
Biotechnology companies to develop new biotechnological products and technologies.

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Next generation sequencing (NGS) is een geavanceerde technologie die het mogelijk maakt om DNA en RNA snel en in grote hoeveelheden te sequencen, waardoor onderzoekers diepgaande inzichten krijgen in genetische informatie.
Zorgverleners, onderzoekers en instellingen die betrokken zijn bij genetisch onderzoek en diagnostiek moeten next generation sequencing indienen.
Next generation sequencing wordt ingevuld door de relevante gegevens te verzamelen, zoals monsterinformatie en sequenceresultaten, en deze in een standaardformulier of platform te uploaden.
Het doel van next generation sequencing is om een diepgaand begrip te krijgen van genetische variaties, ziekten te diagnosticeren en gepersonaliseerde geneeskunde mogelijk te maken.
Op next generation sequencing moet informatie worden gerapporteerd zoals de sequentiegegevens, het type monster, de analysemethode en eventuele klinische bevindingen.
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